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Mycobacterium phage Saal
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Detailed Information for Phage Saal
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySahil Aggarwal
Year Found2011
Location FoundRichmond, VA USA
Finding InstitutionVirginia Commonwealth University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.548611 N, 77.449575 W Map
Discovery NotesThe phage was discovered in moist soil at a depth or approximately 3 inches. The outside air temperature was approximately 75 degrees.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 18, 2012
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage184
Sanger Finishing Reactions2
Genome length (bp)57775
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

Click to View

AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesA typical plaque is clear and relatively smooth. In terms of size, there are both small (approximately 0.5mm) and large (approximately 1.5mm) plaques in a single plate.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Saal_1
(Terminase, small subunit)

Saal_2
(Terminase, large subunit)

Saal_3
(Portal)

Saal_4
(Capsid maturation protease)

Saal_5
(Scaffolding)

Saal_6
(Major capsid)

Saal_7

Saal_8

Saal_9

Saal_10

Saal_11
(Major tail subunit)

Saal_12
(Tail assembly chaperone)

Saal_13
(Tail assembly chaperone)

Saal_14
(Tapemeasure)

Saal_15
(Minor tail protein)

Saal_16
(Minor tail protein)

Saal_17
(Minor tail protein)

Saal_18
(Minor tail protein)

Saal_19
(Minor tail protein, D-ala-D-ala carboxypeptidase)

Saal_20
(Minor tail protein)

Saal_21

Saal_22

Saal_23
(Minor tail protein)

Saal_24
(Minor tail protein)

Saal_25

Saal_26

Saal_27

Saal_28

Saal_29

Saal_30
(Lysin A)

Saal_31
(Lysin B)

Saal_32
(Holin)

Saal_33

Saal_34

Saal_35
(DnaQ-like)

Saal_36

Saal_37

Saal_38

Saal_39

Saal_40

Saal_41

Saal_42

Saal_43
(Integrase, Y-int)

Saal_44
(Toxin in toxin/antitoxin system (RelE-like))

Saal_45
(Antitoxin in toxin/antitoxin system (RelB-like))

Saal_46

Saal_47
(Immunity repressor)

Saal_48

Saal_49
(Phage antirepressor)

Saal_50

Saal_51

Saal_52

Saal_53

Saal_54

Saal_55

Saal_56

Saal_57

Saal_58

Saal_59
(WhiB)

Saal_60

Saal_61
(WhiB)

Saal_62
(HTH DNA binding protein)

Saal_63

Saal_64

Saal_65

Saal_66
(AAA ATPase)

Saal_67
(HNH endonuclease)

Saal_68
(DNA helicase/methylase)

Saal_69

Saal_70

Saal_71

Saal_72

Saal_73
(GIY-YIG homing endonuclease)

Saal_74

Saal_75

Saal_76

Saal_77

Saal_78

Saal_79
(MPME1)

Saal_80

Saal_81

Saal_82

Saal_83

Saal_84

Saal_85

Saal_86

Saal_87

Saal_88

Saal_89

Saal_90

Saal_91

Saal_92

Saal_93

Saal_94

Saal_95

Saal_96

Saal_97

Saal_98

Saal_99
(Glycosyl transferase)

Saal_100
(Glycosyl transferase)

Saal_101
(Glycosyl transferase)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKJ025956
Refseq NumberNC_023580
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload