Mycobacterium phage Harley
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Detailed Information for Phage Harley
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLeanne Pillay
Year Found2015
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.926212 S, 30.895681 E Map
Discovery NotesThe soil was taken from my garden at home, from around the rose bushes.
Naming NotesThis phage is named after my pitbull Harley who passed away earlier this year.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 27, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2848
Genome length (bp)58731
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

BabytheFifth (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

BoomCrackaPop (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Estella (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Kalikimaka (F1)

Katya (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MerrillCO18 (F1)

Mille (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Myosotis (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

Taus (F1)

TDanisky (F1)

TonaldTDrump (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
Plaque NotesPlaques presented themselves as distinct, circular zones of clearing, with clear centers and measured approximately 1.8mm in diameter
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Harley_1
(terminase, small subunit)

Harley_2
(terminase, large subunit)

Harley_3
(portal protein)

Harley_4
(capsid maturation protease)

Harley_5
(scaffolding protein)

Harley_6
(major capsid protein)

Harley_7
(head-to-tail connector complex protein)

Harley_8

Harley_9

Harley_10
(head-to-tail connector complex protein)

Harley_11
(major tail subunit)

Harley_12
(tail assembly chaperone)

Harley_13
(tail assembly chaperone)

Harley_14
(tapemeasure protein)

Harley_15
(minor tail protein)

Harley_16
(minor tail protein)

Harley_17
(minor tail protein)

Harley_18
(minor tail protein)

Harley_19
(minor tail protein, D-ala-D-ala carboxypeptidase)

Harley_20
(minor tail protein)

Harley_21
(minor tail protein)

Harley_22
(minor tail protein)

Harley_23
(minor tail protein)

Harley_24
(minor tail protein)

Harley_25

Harley_26

Harley_27

Harley_28

Harley_29

Harley_30
(lysin A)

Harley_31
(lysin B)

Harley_32
(holin)

Harley_33

Harley_34

Harley_35
(DnaQ-like exonuclease (DNA polymerase III subunit))

Harley_36

Harley_37

Harley_38

Harley_39

Harley_40

Harley_41

Harley_42

Harley_43

Harley_44

Harley_45
(integrase (Y-Int))

Harley_46

Harley_47

Harley_48
(immunity repressor)

Harley_49
(HTH DNA-binding-domain protein)

Harley_50
(antirepressor)

Harley_51

Harley_52

Harley_53

Harley_54

Harley_55

Harley_56

Harley_57

Harley_58

Harley_59

Harley_60
(whiB)

Harley_61

Harley_62

Harley_63

Harley_64

Harley_65

Harley_66

Harley_67
(HNH endonuclease)

Harley_68
(DNA methylase, HindIII-like)

Harley_69

Harley_70

Harley_71

Harley_72

Harley_73

Harley_74

Harley_75

Harley_76

Harley_77

Harley_78

Harley_79

Harley_80

Harley_81

Harley_82

Harley_83

Harley_84

Harley_85

Harley_86

Harley_87

Harley_88

Harley_89

Harley_90

Harley_91

Harley_92

Harley_93

Harley_94

Harley_95

Harley_96

Harley_97
(HTH DNA binding protein, MerR-like)

Harley_98

Harley_99

Harley_100

Harley_101

Harley_102
(glycosyltransferase)

Harley_103

Harley_104
(serine/threonine kinase)

Harley_105

Harley_106

Harley_107
(glycosyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH632119
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload