Mycobacterium phage Priscilla
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Detailed Information for Phage Priscilla
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLaurie Gene Seifert and Eugene Kim
Year Found2015
Location FoundNyack, NY USA
Finding InstitutionNyack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates41.081081 N, 73.926653 W Map
Discovery NotesSoil was soft, moist black-brown dirt. It also had debris. Soil site had surrounding trees with a lot of roots in the soil. Sample was collected behind Dunbar Hall.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 24, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage375
Genome length (bp)58230
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

BabytheFifth (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

BoomCrackaPop (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Kalikimaka (F1)

Katya (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MerrillCO18 (F1)

Mille (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Myosotis (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

Taus (F1)

TDanisky (F1)

TonaldTDrump (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
Plaque NotesPlaque Morphology (after 24hrs):
Diameter: 1-2mm
Clarity of Plaque: Clear
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Priscilla_1
(terminase small subunit)

Priscilla_2
(terminase large subunit)

Priscilla_3
(portal protein)

Priscilla_4
(capsid maturation protease)

Priscilla_5
(scaffolding protein)

Priscilla_6
(major capsid protein)

Priscilla_7
(head-to-tail connector complex protein)

Priscilla_8
(head-to-tail connector complex protein)

Priscilla_9

Priscilla_10
(head-to-tail connector complex protein)

Priscilla_11
(major tail protein)

Priscilla_12
(tail assembly chaperone)

Priscilla_13
(tail assembly chaperone)

Priscilla_14
(tape measure protein)

Priscilla_15
(minor tail protein)

Priscilla_16
(minor tail protein)

Priscilla_17
(minor tail protein)

Priscilla_18
(minor tail protein)

Priscilla_19
(minor tail protein)

Priscilla_20
(minor tail protein)

Priscilla_21
(minor tail protein)

Priscilla_22
(minor tail protein)

Priscilla_23
(minor tail protein)

Priscilla_24
(minor tail protein)

Priscilla_25
(HTH DNA binding protein)

Priscilla_26

Priscilla_27

Priscilla_28
(membrane protein)

Priscilla_29

Priscilla_30
(lysin A)

Priscilla_31
(lysin B)

Priscilla_32
(holin)

Priscilla_33
(membrane protein)

Priscilla_34

Priscilla_35
(DNA polymerase III subunit)

Priscilla_36

Priscilla_37
(membrane protein)

Priscilla_38
(membrane protein)

Priscilla_39
(DNA binding protein)

Priscilla_40

Priscilla_41
(transposase)

Priscilla_42

Priscilla_43

Priscilla_44
(integrase)

Priscilla_45
(membrane protein)

Priscilla_46
(immunity repressor)

Priscilla_47
(CRO protein)

Priscilla_48

Priscilla_49

Priscilla_50
(excise)

Priscilla_51

Priscilla_52

Priscilla_53

Priscilla_54

Priscilla_55

Priscilla_56

Priscilla_57
(WhiB family transcription factor)

Priscilla_58

Priscilla_59
(WhiB family transcription factor)

Priscilla_60
(helix-turn-helix DNA binding domain protein)

Priscilla_61

Priscilla_62

Priscilla_63

Priscilla_64
(DNA recombinase)

Priscilla_65
(HNH endonuclease)

Priscilla_66
(DNA helicase)

Priscilla_67
(DNA methylase)

Priscilla_68
(membrane protein)

Priscilla_69

Priscilla_70

Priscilla_71
(G-I-Y Y-I-G endonuclease)

Priscilla_72
(helix-turn-helix DNA binding domain protein)

Priscilla_73

Priscilla_74

Priscilla_75

Priscilla_76

Priscilla_77

Priscilla_78
(MPME 1 protein)

Priscilla_79

Priscilla_80

Priscilla_81

Priscilla_82

Priscilla_83

Priscilla_84

Priscilla_85

Priscilla_86

Priscilla_87

Priscilla_88

Priscilla_89

Priscilla_90

Priscilla_91

Priscilla_92
(G-I-Y Y-I-G endonuclease)

Priscilla_93

Priscilla_94

Priscilla_95
(membrane protein)

Priscilla_96

Priscilla_97

Priscilla_98

Priscilla_99

Priscilla_100

Priscilla_101

Priscilla_102
(glycosyltransferase)

Priscilla_103

Priscilla_104
(glycosyltransferase)

Priscilla_105
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG872841
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.2x10^11 pfu/mL
Date of SEA Lysate Titering Oct 27, 2015
Pitt Freezer Box# 22
Pitt Freezer Box Grid# G11
Available Files
GenBank File for PhameratorDownload