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Mycobacterium phage Kingsley
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Detailed Information for Phage Kingsley
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySteven Black
Year Found2015
Location FoundBlacksburg, VA USA
Finding InstitutionVirginia Tech
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.24731 N, 80.4175 W Map
Discovery NotesFrom Potted Plant (genus Schlumbergera)
Plant had sat outside for a month, then was moved inside for over a month before the sample was taken.
Naming NotesI just thought it sounded good
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 10, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1576
Genome length (bp)60300
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGATGGCAT
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

Click to View

AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque Notesvarying sizes of plaques, but all are very small and clear
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

Kingsley_1
(HNH endonuclease)

Kingsley_2
(terminase small subunit)

Kingsley_3
(terminase large subunit)

Kingsley_4
(portal protein)

Kingsley_5
(capsid maturation protease)

Kingsley_6
(scaffolding protein)

Kingsley_7
(major capsid protein)

Kingsley_8
(head-to-tail connector protein)

Kingsley_9
(head-to-tail connector protein)

Kingsley_10
(head-to-tail connector protein)

Kingsley_11
(head-to-tail connector protein)

Kingsley_12
(major tail protein)

Kingsley_13
(tail assembly chaperone)

Kingsley_14
(tail assembly chaperone)

Kingsley_15
(tape measure protein)

Kingsley_16
(minor tail protein)

Kingsley_17
(minor tail protein)

Kingsley_18
(minor tail protein)

Kingsley_19
(minor tail protein)

Kingsley_20
(minor tail protein)

Kingsley_21
(minor tail protein)

Kingsley_22

Kingsley_23
(helix-turn-helix DNA binding domain protein)

Kingsley_24

Kingsley_25

Kingsley_26
(membrane protein)

Kingsley_27

Kingsley_28
(lysin A)

Kingsley_29
(HNH endonuclease)

Kingsley_30
(lysin B)

Kingsley_31
(holin)

Kingsley_32
(membrane protein)

Kingsley_33

Kingsley_34
(DNA polymerase III subunit)

Kingsley_35

Kingsley_36

Kingsley_37
(membrane protein)

Kingsley_38
(integrase)

Kingsley_39
(membrane protein)

Kingsley_40
(immunity repressor)

Kingsley_41
(CRO protein)

Kingsley_42
(antirepressor)

Kingsley_43

Kingsley_44

Kingsley_45

Kingsley_46

Kingsley_47
(excise)

Kingsley_48

Kingsley_49
(membrane protein)

Kingsley_50

Kingsley_51

Kingsley_52

Kingsley_53
(membrane protein)

Kingsley_54
(WhiB family transcription factor)

Kingsley_55
(HTH DNA binding protein)

Kingsley_56
(HTH DNA binding protein)

Kingsley_57
(WhiB family transcription factor)

Kingsley_58

Kingsley_59

Kingsley_60

Kingsley_61

Kingsley_62
(DNA recombinase)

Kingsley_63
(HNH endonuclease)

Kingsley_64
(DNA helicase/methylase)

Kingsley_65
(membrane protein)

Kingsley_66

Kingsley_67
(HTH DNA binding protein)

Kingsley_68

Kingsley_69

Kingsley_70
(DNA cytosine methlyase)

Kingsley_71
(DNA cytosine methlyase)

Kingsley_72
(DNA binding protein)

Kingsley_73

Kingsley_74

Kingsley_75

Kingsley_76
(DNA methylase)

Kingsley_77

Kingsley_78

Kingsley_79

Kingsley_80

Kingsley_81

Kingsley_82

Kingsley_83

Kingsley_84

Kingsley_85

Kingsley_86

Kingsley_87

Kingsley_88

Kingsley_89

Kingsley_90

Kingsley_91

Kingsley_92

Kingsley_93

Kingsley_94

Kingsley_95

Kingsley_96

Kingsley_97

Kingsley_98

Kingsley_99

Kingsley_100
(GIY-YIG endonuclease)

Kingsley_101

Kingsley_102

Kingsley_103

Kingsley_104

Kingsley_105
(membrane protein)

Kingsley_106

Kingsley_107

Kingsley_108

Kingsley_109

Kingsley_110
(glycosyltransferase)

Kingsley_111

Kingsley_112
(glycosyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKY702574
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload