The Actinobacteriophage Database

Mycobacterium phage DirtMcgirt

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Detailed Information for Phage DirtMcgirt
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	John Brooker
Year Found	2012
Location Found	Charleston, SC USA
Finding Institution	College of Charleston
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	32.782927 N, 79.945389 W Map
Discovery Notes	Found in muddy driveway by puddles of recent flood water almost black in color
Naming Notes	To denote contamination of a set of streaks I labeled them as "Dirt Mcgirt". Upon seeing the label, my instructor noted that she liked my phage name. And so it became my phage name.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 31, 2013
Sequencing Facility	Virginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	260
Sanger Finishing Reactions	2
Genome length (bp)	54396
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CCGATGGCAT
GC Content	61.1%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) MulchSalad (None) Akhila (F1) Alexphander (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5)
Annotating Institution	Rockland Community College
Annotation Status	In GenBank
Plaque Notes	Lytic, 0.5cm plaque diameter.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View DirtMcgirt_1 (HNH endonuclease) DirtMcgirt_2 (terminase small subunit) DirtMcgirt_3 (terminase large subunit) DirtMcgirt_4 (portal protein) DirtMcgirt_5 (capsid maturation protease) DirtMcgirt_6 (scaffolding protein) DirtMcgirt_7 (major capsid protein) DirtMcgirt_8 (head-to-tail adaptor) DirtMcgirt_9 (head-to-tail stopper) DirtMcgirt_10 DirtMcgirt_11 (tail terminator) DirtMcgirt_12 (major tail protein) DirtMcgirt_13 (tail assembly chaperone) DirtMcgirt_14 (tail assembly chaperone) DirtMcgirt_15 (tape measure protein) DirtMcgirt_16 (minor tail protein) DirtMcgirt_17 (minor tail protein) DirtMcgirt_18 (minor tail protein) DirtMcgirt_19 (minor tail protein) DirtMcgirt_20 (minor tail protein) DirtMcgirt_21 DirtMcgirt_22 DirtMcgirt_23 DirtMcgirt_24 DirtMcgirt_25 DirtMcgirt_26 (helix-turn-helix DNA binding domain protein) DirtMcgirt_27 DirtMcgirt_28 DirtMcgirt_29 DirtMcgirt_30 DirtMcgirt_31 (lysin A) DirtMcgirt_32 (lysin B) DirtMcgirt_33 (holin) DirtMcgirt_34 DirtMcgirt_35 DirtMcgirt_36 (DnaQ-like DNA polymerase III subunit) DirtMcgirt_37 DirtMcgirt_38 DirtMcgirt_39 DirtMcgirt_40 DirtMcgirt_41 (tyrosine integrase) DirtMcgirt_42 DirtMcgirt_43 DirtMcgirt_44 (immunity repressor) DirtMcgirt_45 (Cro protein) DirtMcgirt_46 (antirepressor) DirtMcgirt_47 DirtMcgirt_48 DirtMcgirt_49 DirtMcgirt_50 (excise) DirtMcgirt_51 DirtMcgirt_52 DirtMcgirt_53 DirtMcgirt_54 DirtMcgirt_55 DirtMcgirt_56 (WhiB family transcription factor) DirtMcgirt_57 (helix-turn-helix DNA binding domain protein) DirtMcgirt_58 (WhiB family transcription factor) DirtMcgirt_59 (helix-turn-helix DNA binding domain protein) DirtMcgirt_60 DirtMcgirt_61 DirtMcgirt_62 DirtMcgirt_63 DirtMcgirt_64 (HNH endonuclease) DirtMcgirt_65 DirtMcgirt_66 DirtMcgirt_67 DirtMcgirt_68 DirtMcgirt_69 (PAPS reductase-like domain protein) DirtMcgirt_70 DirtMcgirt_71 (G-I-Y Y-I-G endonuclease) DirtMcgirt_72 (helix-turn-helix DNA binding domain protein) DirtMcgirt_73 DirtMcgirt_74 DirtMcgirt_75 DirtMcgirt_76 DirtMcgirt_77 DirtMcgirt_78 DirtMcgirt_79 DirtMcgirt_80 DirtMcgirt_81 DirtMcgirt_82 DirtMcgirt_83 DirtMcgirt_84 DirtMcgirt_85 DirtMcgirt_86 DirtMcgirt_87 DirtMcgirt_88 DirtMcgirt_89 DirtMcgirt_90 DirtMcgirt_91 DirtMcgirt_92 DirtMcgirt_93 DirtMcgirt_94 DirtMcgirt_95 (MerR-like helix-turn-helix DNA binding domain protein) DirtMcgirt_96 DirtMcgirt_97 DirtMcgirt_98 DirtMcgirt_99 DirtMcgirt_100 DirtMcgirt_101 (glycosyltransferase) DirtMcgirt_102 DirtMcgirt_103 DirtMcgirt_104 (glycosyltransferase)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MZ681512
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	34
Pitt Freezer Box Grid#	47
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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