Mycobacterium phage Mutaforma13
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Detailed Information for Phage Mutaforma13
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHannah Huntley
Year Found2010
Location FoundRaleigh, NC USA
Finding InstitutionNorth Carolina State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates35.782161 N, 78.666205 W Map
Discovery NotesI collected my soil sample on the morning of August 24th, 2010. It was a cool morning, and it was lightly raining, therefore my soil sample was cool and moist. The air temperature was about 70 degrees.

The soil was under a thin layer of mulch, and I collected my sample from about an inch under the soil surface.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 22, 2011
Genome length (bp)57701
Character of genome ends3' Sticky Overhang
Overhang Length10 bp
Overhang SequenceCGGTAGGCGC
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

Click to View

AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesAt high concentrations, my phage seems to exhibit characteristics
of a temperate life cycle. The plaques are small (1-2 mm in diameter) and turbid.

At lower concentrations, however, my phage seems to have a lytic life cycle. The
plaques are larger (3 mm in diameter) and are clear and round. They also have a
bulls-eye ring around them.

I think that the variation in plaque morphology may be due an ability of the phage
to communicate with others of its kind through chemical signaling. Maybe its life
cycle and replication rate are controlled by a protein that it secretes depending on
the concentration of itself in its environment.
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes105
Has been Phamerated?Yes
Gene List

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Mutaforma13_1
(terminase, small subunit)

Mutaforma13_2
(Terminase, large subunit)

Mutaforma13_3
(Portal)

Mutaforma13_4
(capsid maturation protease)

Mutaforma13_5
(Scaffold)

Mutaforma13_6
(Major Capsid)

Mutaforma13_7

Mutaforma13_8

Mutaforma13_9

Mutaforma13_10

Mutaforma13_11
(Major tail subunit)

Mutaforma13_12
(Tail assembly chaperone)

Mutaforma13_13
(tail assembly chaperone)

Mutaforma13_14
(Tapemeasure)

Mutaforma13_15
(minor tail subunit)

Mutaforma13_16
(minor tail subunit)

Mutaforma13_17
(minor tail subunit)

Mutaforma13_18
(minor tail subunit)

Mutaforma13_19
(D-ala-D-ala carboxypeptidase)

Mutaforma13_20

Mutaforma13_21

Mutaforma13_22

Mutaforma13_23

Mutaforma13_24

Mutaforma13_25

Mutaforma13_26

Mutaforma13_27

Mutaforma13_28

Mutaforma13_29

Mutaforma13_30
(LysA)

Mutaforma13_31
(LysB)

Mutaforma13_32
(holin)

Mutaforma13_33

Mutaforma13_34

Mutaforma13_35
(DnaQ-like)

Mutaforma13_36

Mutaforma13_37

Mutaforma13_38

Mutaforma13_39

Mutaforma13_40

Mutaforma13_41

Mutaforma13_42

Mutaforma13_43

Mutaforma13_44

Mutaforma13_45
(Integrase (Y-INT))

Mutaforma13_46

Mutaforma13_47

Mutaforma13_48
(DNA binding domain (XRE family))

Mutaforma13_49
(antirepressor)

Mutaforma13_50

Mutaforma13_51

Mutaforma13_52

Mutaforma13_53

Mutaforma13_54
(HNH endonuclease domain)

Mutaforma13_55

Mutaforma13_56

Mutaforma13_57

Mutaforma13_58

Mutaforma13_59

Mutaforma13_60

Mutaforma13_61
(WhiB)

Mutaforma13_62

Mutaforma13_63
(WhiB)

Mutaforma13_64
(helix-turn-helix DNA binding domain)

Mutaforma13_65

Mutaforma13_66

Mutaforma13_67

Mutaforma13_68

Mutaforma13_69
(HNH Endonuclease domain)

Mutaforma13_70

Mutaforma13_71
(HNH homing endonuclease domain)

Mutaforma13_72

Mutaforma13_73

Mutaforma13_74

Mutaforma13_75

Mutaforma13_76

Mutaforma13_77

Mutaforma13_78

Mutaforma13_79

Mutaforma13_80

Mutaforma13_81

Mutaforma13_82

Mutaforma13_83

Mutaforma13_84

Mutaforma13_85

Mutaforma13_86

Mutaforma13_87

Mutaforma13_88

Mutaforma13_89

Mutaforma13_90

Mutaforma13_91

Mutaforma13_92

Mutaforma13_93

Mutaforma13_94
(GIY-YIG homing endonuclease)

Mutaforma13_95

Mutaforma13_96

Mutaforma13_97

Mutaforma13_98

Mutaforma13_99

Mutaforma13_100

Mutaforma13_101

Mutaforma13_102
(polypeptide N-acetylgalactosaminyl transferase)

Mutaforma13_103

Mutaforma13_104
(Ser/Thr Kinase)

Mutaforma13_105
(N-acetylglucosaminyl transferase)

Mutaforma13_106
(HNH Endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJN020142
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload