Mycobacterium phage Nimbo
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Detailed Information for Phage Nimbo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByVictoria Palochik
Year Found2017
Location FoundPhiladelphia, PA USA
Finding InstitutionDrexel University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature26°C
GPS Coordinates39.96 N, 75.19 W Map
Discovery NotesThe soil sample was taken from one of the trees planted in the sidewalk outside of the WW Hagerty Library on Drexel University's campus.
Naming NotesThe phage was named after the cumulonimbus cloud. When the phage was on a plate with a low serial dilution, the plaques that would form would overlap. Like cumulonimbus clouds, I found you could make shapes and figures out of the overlapping, therefore sparking inspiration for the name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 31, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1182
Genome length (bp)55767
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

BabytheFifth (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

BoomCrackaPop (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Estella (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Kalikimaka (F1)

Katya (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MerrillCO18 (F1)

Mille (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Ms6 (F1)

Mutaforma13 (F1)

Myosotis (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

Taus (F1)

TDanisky (F1)

TonaldTDrump (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
Plaque NotesPlaques consisted of clear perfect circles approximately 0.30 mm in diameter with smooth margins. Also, the plaques has consistent morphology throughout the lab.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Nimbo_1
(terminase small subunit)

Nimbo_2
(terminase large subunit)

Nimbo_3
(portal protein)

Nimbo_4
(capsid maturation protease)

Nimbo_5
(scaffolding protein)

Nimbo_6
(major capsid protein)

Nimbo_7
(head-to-tail-connector complex protein)

Nimbo_8
(head-to-tail-connector complex protein)

Nimbo_9

Nimbo_10
(head-to-tail-connector complex protein)

Nimbo_11
(major tail protein)

Nimbo_12
(tail assembly chaperone)

Nimbo_13
(tail assembly chaperone)

Nimbo_14
(tape measure protein)

Nimbo_15
(minor tail protein)

Nimbo_16
(minor tail protein)

Nimbo_17

Nimbo_18
(minor tail protein)

Nimbo_19
(minor tail protein)

Nimbo_20

Nimbo_21

Nimbo_22

Nimbo_23

Nimbo_24

Nimbo_25

Nimbo_26

Nimbo_27

Nimbo_28

Nimbo_29

Nimbo_30
(lysin A)

Nimbo_31
(lysin B)

Nimbo_32
(holin)

Nimbo_33

Nimbo_34

Nimbo_35
(DnaQ-like exonuclease)

Nimbo_36

Nimbo_37

Nimbo_38

Nimbo_39
(excise)

Nimbo_40
(integrase)

Nimbo_41

Nimbo_42
(immunity repressor)

Nimbo_43
(Cro protein)

Nimbo_44

Nimbo_45
(excise)

Nimbo_46

Nimbo_47

Nimbo_48

Nimbo_49

Nimbo_50

Nimbo_51
(WhiB family transcription factor)

Nimbo_52

Nimbo_53
(WhiB family transcription factor)

Nimbo_54

Nimbo_55

Nimbo_56

Nimbo_57
(DNA recombinase)

Nimbo_58

Nimbo_59
(HNH endonuclease)

Nimbo_60

Nimbo_61

Nimbo_62

Nimbo_63
(DNA methylase)

Nimbo_64

Nimbo_65

Nimbo_66
(DNA methylase)

Nimbo_67

Nimbo_68

Nimbo_69

Nimbo_70

Nimbo_71

Nimbo_72

Nimbo_73

Nimbo_74

Nimbo_75

Nimbo_76

Nimbo_77

Nimbo_78

Nimbo_79

Nimbo_80

Nimbo_81

Nimbo_82

Nimbo_83

Nimbo_84

Nimbo_85

Nimbo_86
(MerR-like HTH DNA binding protein)

Nimbo_87

Nimbo_88

Nimbo_89

Nimbo_90

Nimbo_91

Nimbo_92

Nimbo_93
(glycosyltransferase)

Nimbo_94

Nimbo_95
(glycosyltransferase)

Nimbo_96
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH669009
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.2*10^11 pfu/mL
Pitt Freezer Box# 60
Pitt Freezer Box Grid# H7
Available Files
GenBank File for PhameratorDownload