Mycobacterium phage Nimbo
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Detailed Information for Phage Nimbo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByVictoria Palochik
Year Found2017
Location FoundPhiladelphia, PA USA
Finding InstitutionDrexel University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature26°C
GPS Coordinates39.96 N, 75.19 W Map
Discovery NotesThe soil sample was taken from one of the trees planted in the sidewalk outside of the WW Hagerty Library on Drexel University's campus.
Naming NotesThe phage was named after the cumulonimbus cloud. When the phage was on a plate with a low serial dilution, the plaques that would form would overlap. Like cumulonimbus clouds, I found you could make shapes and figures out of the overlapping, therefore sparking inspiration for the name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 31, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1182
Genome length (bp)55767
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesPlaques consisted of clear perfect circles approximately 0.30 mm in diameter with smooth margins. Also, the plaques has consistent morphology throughout the lab.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet