Mycobacterium phage Wachhund
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage Wachhund
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDimond Shelton
Year Found2016
Location FoundCromwell, KY United States of America
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates37.351064 N, 86.77467 W Map
Discovery NotesI gathered this sample from my dog's pen at 7:16AM on September 12, 2016. I dug down approximately 2.5 inches when gathering my sample. There was minimal direct sunlight in this area, and the ground temperature was 12.2°C. The soil was moist.
Naming NotesSince I gathered this sample from my dog's pen, I wanted to name my phage something that reminded me of where I found my sample. My dog is a purebred German Shepherd and was trained to protect our property. Wachhund is German for "guard dog."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 30, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2423
Genome length (bp)54513
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Other Cluster Members

Click to View

AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

BuzzLyseyear (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Emma (F1)

Empress (F1)

Estave1 (F1)

Estella (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

Judylove (F1)

Kalikimaka (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LillyLatzon (F1)

Llama (F1)

Llij (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Mutaforma13 (F1)

Nemain (F1)

Nitzel (F1)

Noodle (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Polka14 (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

Cornie (F5)

Plaque NotesMy plaques are moderately small and extremely clear on plates, but exhibit faint halos around the clear plaque center. The clear plaque in the center is 1 millimeter in diameter with a turbid halo 2 millimeters in radius surrounding. These halos were only visible out to the 10^-8 dilution. At lower dilutions, the phage appeared to be lytic. As the space between individual plaques increased at higher dilutions, "bull's eyes" could be seen around the clear plaques. Plates were, typically, incubated at 30°C for 48 hours.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

Wachhund_1
(terminase small subunit)

Wachhund_2
(terminase large subunit)

Wachhund_3
(portal protein)

Wachhund_4
(capsid maturation protease)

Wachhund_5
(scaffolding protein)

Wachhund_6
(major capsid protein)

Wachhund_7
(head-to-tail connector protein)

Wachhund_8
(head-to-tail connector protein)

Wachhund_9
(head-to-tail connector protein)

Wachhund_10
(head-to-tail connector protein)

Wachhund_11
(major tail protein)

Wachhund_12
(tail assembly chaperone)

Wachhund_13
(tail assembly chaperone)

Wachhund_14
(tape measure protein)

Wachhund_15
(minor tail protein)

Wachhund_16
(minor tail protein)

Wachhund_17
(minor tail protein)

Wachhund_18
(minor tail protein)

Wachhund_19
(minor tail protein)

Wachhund_20
(minor tail protein)

Wachhund_21
(minor tail protein)

Wachhund_22
(minor tail protein)

Wachhund_23
(minor tail protein)

Wachhund_24
(minor tail protein)

Wachhund_25
(helix-turn-helix DNA binding domain protein)

Wachhund_26

Wachhund_27

Wachhund_28
(membrane protein)

Wachhund_29

Wachhund_30
(lysin A)

Wachhund_31
(lysin B)

Wachhund_32
(holin)

Wachhund_33
(membrane protein)

Wachhund_34

Wachhund_35
(DNA polymerase III subunit)

Wachhund_36

Wachhund_37
(membrane protein)

Wachhund_38

Wachhund_39
(membrane protein)

Wachhund_40

Wachhund_41

Wachhund_42

Wachhund_43

Wachhund_44
(integrase)

Wachhund_45
(membrane protein)

Wachhund_46

Wachhund_47
(immunity repressor)

Wachhund_48
(CRO protein)

Wachhund_49
(antirepressor)

Wachhund_50

Wachhund_51

Wachhund_52
(excise)

Wachhund_53

Wachhund_54
(membrane protein)

Wachhund_55

Wachhund_56

Wachhund_57

Wachhund_58
(membrane protein)

Wachhund_59
(WhiB family transcription factor)

Wachhund_60

Wachhund_61
(transposase)

Wachhund_62

Wachhund_63

Wachhund_64

Wachhund_65
(AAA ATPase)

Wachhund_66
(HNH endonuclease)

Wachhund_67
(membrane protein)

Wachhund_68

Wachhund_69
(DNA methylase)

Wachhund_70

Wachhund_71

Wachhund_72

Wachhund_73

Wachhund_74

Wachhund_75

Wachhund_76

Wachhund_77

Wachhund_78

Wachhund_79

Wachhund_80

Wachhund_81

Wachhund_82

Wachhund_83

Wachhund_84

Wachhund_85

Wachhund_86

Wachhund_87

Wachhund_88

Wachhund_89

Wachhund_90

Wachhund_91

Wachhund_92
(MerR-like HTH DNA binding protein)

Wachhund_93

Wachhund_94

Wachhund_95

Wachhund_96

Wachhund_97
(glycosyltransferase)

Wachhund_98

Wachhund_99
(serine/threonine kinase)

Wachhund_100
(glycosyltransferase)

Wachhund_101
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF668287
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload