Mycobacterium phage Cerasum
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Detailed Information for Phage Cerasum
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLaura Krings and Jessica Kellerstrass
Year Found2013
Location FoundKenosha, WI United States
Finding InstitutionCarthage College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.621111 N, 87.820556 W Map
Discovery NotesThe soil sample was taken from a flower garden outside of Tarble Hall on the Carthage College campus. The flower garden is located directly in front of Tarble's front doors. The soil sample was moist and compact, found in between flowering plants at a depth of approximately 3.8 cm. The temperature was 20 degrees C.
Naming NotesThe name Cerasum is the Latin word for 'cherry.' I selected Cerasum for the phages name because after seeing the phage on the electron microscope, I thought it looked like a cherry: containing a long tail (stem) and a circular capsid (cherry). Hence the name Cerasum.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2014
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage442
Sanger Finishing Reactions7
Genome length (bp)53636
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGATGGCAT
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

BabytheFifth (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

BoomCrackaPop (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Estella (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Kalikimaka (F1)

Katya (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MerrillCO18 (F1)

Mille (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Mozy (F1)

Ms6 (F1)

Mutaforma13 (F1)

Myosotis (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

Taus (F1)

TDanisky (F1)

TonaldTDrump (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
Plaque NotesPhage has hazy plaques, leading to the conclusion that it is a temperate phage. Each plaque contains halos around them. The morphology of the plaques has three different sizes:
90% are 0.20 cm
5% are 0.12 cm
5% are 0.08 cm
MorphotypeSiphoviridae
Number of Genes102
Has been Phamerated?Yes
Gene List

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Cerasum_1
(HNH endonuclease)

Cerasum_2
(terminase small subunit)

Cerasum_3
(terminase large subunit)

Cerasum_4
(portal protein)

Cerasum_5
(capsid maturation protease)

Cerasum_6
(scaffolding protein)

Cerasum_7
(major capsid protein)

Cerasum_8
(head-to-tail connector protein)

Cerasum_9
(head-to-tail connector protein)

Cerasum_10

Cerasum_11

Cerasum_12
(major tail subunit)

Cerasum_13
(tail assembly chaperone)

Cerasum_14
(tail assembly chaperone)

Cerasum_15
(tapemeasure)

Cerasum_16
(minor tail protein)

Cerasum_17
(minor tail protein)

Cerasum_18
(minor tail protein)

Cerasum_19
(minor tail protein)

Cerasum_20

Cerasum_21

Cerasum_22
(minor tail protein)

Cerasum_23

Cerasum_24

Cerasum_25

Cerasum_26
(lysin A)

Cerasum_27
(lysin B)

Cerasum_28
(holin)

Cerasum_29

Cerasum_30

Cerasum_31
(Dna-Q like exonuclease)

Cerasum_32

Cerasum_33

Cerasum_34

Cerasum_35

Cerasum_36

Cerasum_37

Cerasum_38
(integrase)

Cerasum_39

Cerasum_40
(immunity repressor)

Cerasum_41
(CRO protein)

Cerasum_42
(DNA damage inducible protein D-like protein)

Cerasum_43
(HTH domain DNA binding protein)

Cerasum_44

Cerasum_45

Cerasum_46
(Xis)

Cerasum_47

Cerasum_48

Cerasum_49

Cerasum_50

Cerasum_51

Cerasum_52

Cerasum_53
(WhiB)

Cerasum_54
(HTH DNA binding protein)

Cerasum_55
(HTH DNA binding protein)

Cerasum_56
(WhiB)

Cerasum_57

Cerasum_58

Cerasum_59

Cerasum_60

Cerasum_61
(RecA)

Cerasum_62
(HNH endonuclease)

Cerasum_63
(DNA helicase/methylase)

Cerasum_64

Cerasum_65

Cerasum_66

Cerasum_67

Cerasum_68

Cerasum_69

Cerasum_70

Cerasum_71

Cerasum_72

Cerasum_73

Cerasum_74

Cerasum_75

Cerasum_76

Cerasum_77

Cerasum_78

Cerasum_79

Cerasum_80

Cerasum_81

Cerasum_82

Cerasum_83

Cerasum_84

Cerasum_85

Cerasum_86

Cerasum_87

Cerasum_88

Cerasum_89

Cerasum_90
(GIY-YIG endonuclease)

Cerasum_91

Cerasum_92

Cerasum_93

Cerasum_94

Cerasum_95

Cerasum_96

Cerasum_97

Cerasum_98

Cerasum_99

Cerasum_100
(polypeptide-N-acetylgalactosaminyltransferase)

Cerasum_101

Cerasum_102
(glycosyltransferase)

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKM215148
Refseq NumberNC_026604
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.8 X 10 ^10
Date of SEA Lysate Titering Oct 30, 2013
Pitt Freezer Box# 4
Pitt Freezer Box Grid# G9
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload