| Detailed Information for Phage Achebe |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Grace Babbs |
| Year Found | 2012 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.98543 N, 86.449006 W Map |
| Discovery Notes | My phage was discovered from soil isolated from WKU's campus under an oak tree approximately 8 cm from the tree. The dark, smooth sample was collected from a depth of 2.5 cm. |
| Naming Notes | Achebe is named for the Nigerian novelist Chinua Achebe. He wrote the acclaimed novel 'Things Fall Apart', and this phage is quite fragile. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 520 |
| Sanger Finishing Reactions | 3 |
| Genome length (bp) | 51433 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTTA |
| GC Content | 63.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | My phage forms relatively small (~2mm), clear plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 86 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KY083059 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.4*10^10 pfu's |
| Pitt Freezer Box# |
43 |
| Pitt Freezer Box Grid# |
1 |
| Available Files |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
