Detailed Information for Phage ArcherNM |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Nicole Mahan |
Year Found | 2013 |
Location Found | Pullman, WA USA |
Finding Institution | Washington State University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 46.728969 N, 117.165017 W Map |
Discovery Notes | The soil was damp when collected and the temperature was about 65 degrees Fahrenheit. the soil was obtained from about two inches from the surface and it was raining. |
Naming Notes | The name ArcherNM came from me being an archery hunter and this passion is very close to my heart. The NM is for my initials. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | May 22, 2015 |
Sequencing Facility | The McDonnell Genome Institute at Washington University |
Shotgun Sequencing Method | Pacific Biosciences Sequencing |
Genome length (bp) | 52561 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGTCGGTTA |
GC Content | 64.2% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A2 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | The morphology of the phage found is slightly turbid and is about 2.5 millimeters in size. the plaques also shows the signs of a comet tail. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KU761559 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
Julie Stanton |
SEA Lysate Titer |
1.2*10^11 pfu/ml |
Pitt Freezer Box# |
4 |
Pitt Freezer Box Grid# |
A2 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |