| Detailed Information for Phage ArcherNM |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nicole Mahan |
| Year Found | 2013 |
| Location Found | Pullman, WA USA |
| Finding Institution | Washington State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 46.728969 N, 117.165017 W Map |
| Discovery Notes | The soil was damp when collected and the temperature was about 65 degrees Fahrenheit. the soil was obtained from about two inches from the surface and it was raining. |
| Naming Notes | The name ArcherNM came from me being an archery hunter and this passion is very close to my heart. The NM is for my initials. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 22, 2015 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Pacific Biosciences Sequencing |
| Genome length (bp) | 52561 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 64.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The morphology of the phage found is slightly turbid and is about 2.5 millimeters in size. the plaques also shows the signs of a comet tail. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU761559 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
Julie Stanton |
| SEA Lysate Titer |
1.2*10^11 pfu/ml |
| Pitt Freezer Box# |
4 |
| Pitt Freezer Box Grid# |
A2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
