| Detailed Information for Phage Popcicle |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Angel Lewis, Melanie Martin |
| Year Found | 2011 |
| Location Found | Madawaska, ME USA |
| Finding Institution | University of Maine, Fort Kent |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 47.352564 N, 68.342267 W Map |
| Discovery Notes | The sample came from 7cm under weeds and grass. There were worms in the ground. The soil was wet and it came from my partner's backyard. The temperature outside that day was 59F.It was collected on September 18, 2011 |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 25, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 190 |
| Sanger Finishing Reactions | 13 |
| Genome length (bp) | 50075 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 64.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | In GenBank |
| Plaque Notes | The size of popcicle was 2-3mm. It was turbid and there was no variation. Popcicle was circular. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH976517 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
MEFK |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
27 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
