Detailed Information for Phage ChipMunk |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Michael Botieri and Katrina Harris |
Year Found | 2011 |
Location Found | Orono, ME USA |
Finding Institution | University of Maine, Honors College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 44.897425 N, 68.668122 W Map |
Discovery Notes | Date: 9/6/2011 Found under a tree (treated for Dutch Elms Disease) in very moist soil next to a fairly busy path. Approximately 50' away from nearest building. Depth: 1-3 cm from surface Temp: 59° F |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Mar 30, 2012 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Ion Torrent Sequencing |
Approximate Shotgun Coverage | 133 |
Genome length (bp) | 53932 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGTCGGTTA |
GC Content | 63.1% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A2 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Initially about 3mm in diameter with turbid plaques. Varies in size with different M. smeg cultures. After 4 weeks, the plaque size had reduced to a diameter of 1 mm. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KU985095 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
2011UMHCchipmunkMBKH |
SEA Lysate Titer |
8.3e7 |
Date of SEA Lysate Titering |
Oct 13, 2011 |
Pitt Freezer Box# |
25 |
Pitt Freezer Box Grid# |
29 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |