| Detailed Information for Phage ChipMunk |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Michael Botieri and Katrina Harris |
| Year Found | 2011 |
| Location Found | Orono, ME USA |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 44.897425 N, 68.668122 W Map |
| Discovery Notes | Date: 9/6/2011
Found under a tree (treated for Dutch Elms Disease) in very moist soil next to a fairly busy path. Approximately 50' away from nearest building.
Depth: 1-3 cm from surface
Temp: 59° F |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 30, 2012 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 133 |
| Genome length (bp) | 53932 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotation Status | In GenBank |
| Plaque Notes | Initially about 3mm in diameter with turbid plaques.
Varies in size with different M. smeg cultures.
After 4 weeks, the plaque size had reduced to a diameter of 1 mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU985095 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011UMHCchipmunkMBKH |
| SEA Lysate Titer |
8.3e7 |
| Date of SEA Lysate Titering |
Oct 13, 2011 |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
29 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
