| Detailed Information for Phage Expelliarmus |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Josue Vasquez |
| Year Found | 2011 |
| Location Found | Grand Rapids, MI USA |
| Finding Institution | Calvin College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.930686 N, 85.589367 W Map |
| Discovery Notes | The soil I used was chunky muck from the plant garden located at Calvin College in front of DeVries Hall. The location in which I extracted the soil was underneath a tree in a relatively human disturbance free area. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 13, 2012 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Genome length (bp) | 52274 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGATGTAA |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A8 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Hampden-Sydney College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are clear and distinguished. They are round and are relatively 3mm to 4mm in size for the large variation. The smaller variation relatively 1mm in size give or take. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524492 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
22 |
| Pitt Freezer Box Grid# |
4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
