| Detailed Information for Phage BeesKnees |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Greg Mastrangelo |
| Year Found | 2013 |
| Location Found | Nyack, NY USA |
| Finding Institution | Nyack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.081909 N, 73.927521 W Map |
| Discovery Notes | Hee Jae Ko found this soil sample by a tree by Simpson Hall at Nyack College. This soil sample had two types of phages named Tuco and Beesknees in it. Eddy Santos worked on Tuco while Beesknees was worked on by Greg Mastrangelo. |
| Naming Notes | Beesknees was picked out randomly |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 20, 2013 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 11403 |
| Genome length (bp) | 51702 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotation Status | In GenBank |
| Plaque Notes | Plagues about 5mm in diameter after 24 hrs incubation at 37 degrees. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG872833 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1x10^10 |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
B9 |
| Available Files |
| GenBank File for Phamerator | Download |