Mycobacterium phage Conquerage

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Detailed Information for Phage Conquerage
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCayce Douthitt
Year Found2015
Location FoundPort Saint Lucie, FL USA
Finding InstitutionIndian River State College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Discovery NotesSoil sample was collected from a man-made canal located in a suburban neighborhood at 2:01 pm on 8/28/15. After a 1/2 inch of sandy surface soil was removed, darker moist peaty soil was revealed and this was collected. The soil collected reached two inches deep. Raccoon tracks and an empty turtle shell was visible nearby, indicating heavy animal traffic. There was a shallow pool of water with greenish scum nearby as well.
Naming NotesThe phage exhibited a high MTL (10^12) so it was deemed a "conqueror" of the plates. Conqueror + phage = Conquerage
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 23, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage400
Genome length (bp)52953
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTCGGTTA
GC Content62.5%
Fasta file available?Available upon entering GPS coordinates
Characterization
ClusterA
SubclusterA9
Cluster Life CycleTemperate
Other Cluster Members

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Afua (None)

Ajaxious (None)

Anonia (None)

BaxterTD (None)

Blazer (None)

BlueWhiteSuper (None)

BrownWilse (None)

BuddyNugget (None)

BuzzKiehl (None)

Chara (None)

Clarabelle (None)

Cornelius42 (None)

Cujo (None)

Dannah (None)

Drogon (None)

EGMO (None)

Fido (None)

Fieldrain (None)

FireFlyEcho (None)

FrannyJoe (None)

Freedman (None)

Geezy (None)

Gracie1 (None)

Hata (None)

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HotBoxer (None)

ILoveMyInterns (None)

Interokirkus (None)

JeanneMarie (None)

JeckyllnHyde (None)

Julio (None)

Kilomegawatt (None)

Krzemiun (None)

LaChupacabra (None)

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LilaGirl (None)

LilMac (None)

Lintoc (None)

LittleDude (None)

McKinnon95 (None)

MelBell (None)

MelJoe (None)

MortyC137 (None)

Nakoi (None)

Noodle17 (None)

Norris007 (None)

Ousia (None)

Pbnep65 (None)

PDonaz (None)

PerryPatch (None)

Phern (None)

Poofy (None)

Pwombat (None)

Raziel (None)

RedJewel (None)

Roetering (None)

Rover (None)

Scooter (None)

Serena (None)

Shayla (None)

Skpmule (None)

SmallSoldier (None)

SnapDragon (None)

SoletiStotinki (None)

SoupAstar (None)

Star (None)

Tala (None)

Terrance (None)

Terrence (None)

Tiff5 (None)

TizzleFlarp (None)

TosSolNia (None)

TreeHugger (None)

UMulchMonster (None)

Unsequencer (None)

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ZheroDRT (None)

Abrogate (A1)

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Bigfoot (A1)

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FourStarNutty (A1)

Francis47 (A1)

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Fushigi (A1)

GageAP (A1)

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Graduation (A1)

Greg (A1)

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Homines (A1)

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ILeeKay (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

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Kanely (A1)

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KBG (A1)

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KingLeonidas (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Littlefoot32 (A1)

Lockley (A1)

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Lopton (A1)

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Magnito (A1)

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Marcell (A1)

Maroc7 (A1)

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Michley (A1)

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Moter17 (A1)

MPlant7149 (A1)

MrGordo (A1)

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Mule (A1)

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Oxente (A1)

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Pepe (A1)

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Pinto (A1)

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Sibs6 (A1)

SkiPole (A1)

Smairt (A1)

Smeagol (A1)

Snazzy (A1)

Solon (A1)

SpikeBT (A1)

Spite (A1)

SunTzu (A1)

SwissCheese (A1)

Switzer (A1)

Target (A1)

Tasp14 (A1)

TechBARfly (A1)

TheloniousMonk (A1)

Thor (A1)

Tommy (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

U2 (A1)

Uffda (A1)

Venus (A1)

Violet (A1)

Watermelon (A1)

Wheeler (A1)

Widget (A1)

Wilkins (A1)

Zeeba (A1)

Zeeculate (A1)

Zelezo (A1)

Zephyr (A1)

Zeuska (A1)

Blitzkrieg (A10)

Chupacabra (A10)

ElSilvo (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

Lindberg (A10)

OKCentral2016 (A10)

Rebeuca (A10)

RhynO (A10)

Severus (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Hutc2 (A11)

IshmaelIII (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Salz (A11)

Snape (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Phlei (A13)

Jeeves (A14)

Luchador (A14)

Onism (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Nedarya (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Waits (A15)

EagleEye (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

Agape74 (A2)

AnnaL29 (A2)

Anselm (A2)

Aquarose (A2)

ArcherNM (A2)

AsepTech (A2)

Asuzu (A2)

Bactobuster (A2)

Baehexic (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Brooklyn (A2)

Buddy (A2)

Bugsy (A2)

Bulldog (A2)

Centaur (A2)

Changeling (A2)

Charity (A2)

Che12 (A2)

Cheriba (A2)

ChipMunk (A2)

ClumpyY (A2)

CRB1 (A2)

Crucio (A2)

Crush (A2)

D29 (A2)

Dalmatian (A2)

DBQu4n (A2)

Death (A2)

Drake55 (A2)

DudeLittle (A2)

Echild (A2)

ElFluff (A2)

Epoch (A2)

Equemioh13 (A2)

EvilGenius (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

Goethe (A2)

GoZags (A2)

GridersLegacy (A2)

Heffalump (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

Jinga3000 (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kakar (A2)

Kalpine (A2)

Kempella (A2)

Kerberos (A2)

KingSt (A2)

Kupalo (A2)

L5 (A2)

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LadyBird (A2)

Larenn (A2)

Leogania (A2)

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Lincaica11 (A2)

Lokk (A2)

Loser (A2)

Maduna (A2)

Malec (A2)

Masha1295 (A2)

Miko (A2)

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Naji (A2)

NaSiaTalie (A2)

Nemo (A2)

NicoleTera (A2)

Norris (A2)

NothingSpecial (A2)

Odin (A2)

Ph8s (A2)

Phaded (A2)

Pim618 (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Rachaly (A2)

Radar (A2)

Rauana (A2)

RedRock (A2)

Retro23 (A2)

Scorpio7 (A2)

SemperFi (A2)

Serenity (A2)

Shipshape (A2)

SnapTap (A2)

StarStuff (A2)

Stumpy (A2)

Superchunk (A2)

SweetiePie (A2)

Tiberius20 (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

Turbido (A2)

Tyrone (A2)

Updawg (A2)

Whabigail7 (A2)

WideWale (A2)

Woozle13 (A2)

Yahighness (A2)

Zaaboot (A2)

Anthony (A20)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

Assassinator (A3)

AugsMagnumOpus (A3)

BabyRay (A3)

Beauxregard13 (A3)

BreSam8 (A3)

BuzzBuzz (A3)

Bxz2 (A3)

ChainChomp (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

Dumpling (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

FloMo (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Gravata (A3)

Groupthink (A3)

Grum1 (A3)

GtownJaz (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

Isca (A3)

JenCasNa (A3)

JeppNRM (A3)

JHC117 (A3)

Jobu08 (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

LadyPhaGa (A3)

Lambert1 (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

MadMarie (A3)

Mainiac (A3)

Malinsilva (A3)

Margo (A3)

Marie (A3)

Marius (A3)

MarQuardt (A3)

MatrixWest360 (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MoneyMay (A3)

MuchMore (A3)

Noella (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

Ostojic (A3)

P28Green (A3)

Panamaxus (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

Roadbarry13 (A3)

Rockstar (A3)

Romeo (A3)

Sabia (A3)

Sabinator (A3)

SoilDragon (A3)

Soshari (A3)

Spike509 (A3)

Stagni (A3)

Stephen (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wonder (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

Backyardigan (A4)

Bacon (A4)

Badger (A4)

Bartimeaus (A4)

BellusTerra (A4)

Blackmoor (A4)

Bombshell (A4)

Broseidon (A4)

Bruiser (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Cici (A4)

Cindaradix (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

DAgoa1 (A4)

Datway (A4)

DCNugh (A4)

Deano (A4)

Decimator (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Flapjack (A4)

Florean (A4)

Flux (A4)

Funston (A4)

FuZGrapes (A4)

Gadost (A4)

Gaius (A4)

HamSlice (A4)

Heisenburg (A4)

Holli (A4)

Houdini22 (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Kratark (A4)

Kyee (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

LittleB (A4)

LittleGuy (A4)

Lorenzo (A4)

LucyGoose (A4)

Massacre (A4)

Maverick (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Milton (A4)

Miramae (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nebs (A4)

Nemo27 (A4)

NorthStar (A4)

NotAPhaseMom (A4)

Nyxis (A4)

Obama12 (A4)

Ohfah (A4)

Palestino (A4)

Peaches (A4)

PeterPeter (A4)

Phacado (A4)

Phighter1804 (A4)

Phontbonne (A4)

Pipcraft (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Ruin (A4)

Sabertooth (A4)

Shaka (A4)

SheldonCooper (A4)

Skipitt (A4)

SophiesChoice (A4)

Stasia (A4)

Thanksgivukkah (A4)

TimCurry (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

Tpap (A4)

TygerBlood (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Barbarella (A5)

Benedict (A5)

Bluefalcon (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Dublin (A5)

Electri (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Midas2 (A5)

Naca (A5)

Phlorence (A5)

Scorpia (A5)

Swirley (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

Trey (A5)

UnionJack (A5)

WootieJr (A5)

Yolo (A5)

Artemis2UCLA (A6)

Blue7 (A6)

CloudWang3 (A6)

DaVinci (A6)

EricB (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Hexamo (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Koko (A6)

McFly (A6)

Paper (A6)

Pmask (A6)

Priamo (A6)

SuperAwesome (A6)

ToneTone (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Cyrano (A8)

Dixon (A8)

Expelliarmus (A8)

Fredward (A8)

Kirpan (A8)

Machaera (A8)

Mitchell (A8)

NearlyHeadless (A8)

Roary (A8)

Saintus (A8)

Shresh (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

DreamTeam1 (A9)

Eidsmoe (A9)

Elephantoon (A9)

ExplosioNervosa (A9)

HortumSL17 (A9)

Keziacharles14 (A9)

Klaus (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

Noelle (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

ShadowHunter (A9)

SheaKeira (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotation StatusIn GenBank
Plaque NotesThe phage plaques are small to medium sized. The plaques are clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Conquerage_1

Conquerage_2

Conquerage_3

Conquerage_4
(HNH endonuclease)

Conquerage_5

Conquerage_6
(minor tail protein)

Conquerage_7

Conquerage_8

Conquerage_10

Conquerage_11
(lysin A)

Conquerage_12
(holin)

Conquerage_13
(terminase)

Conquerage_14
(portal protein)

Conquerage_15
(capsid maturation protease)

Conquerage_16
(scaffolding protein)

Conquerage_17
(major capsid protein)

Conquerage_18

Conquerage_19
(head-to-tail connector complex protein)

Conquerage_20

Conquerage_21
(head-to-tail connector complex protein)

Conquerage_22

Conquerage_23
(head-to-tail connector complex protein)

Conquerage_24
(major tail protein)

Conquerage_25
(tail assembly chaperone)

Conquerage_26
(tail assembly chaperone)

Conquerage_27
(tape measure protein)

Conquerage_28
(minor tail protein)

Conquerage_29

Conquerage_30
(minor tail protein)

Conquerage_31

Conquerage_32
(minor tail protein)

Conquerage_33
(minor tail protein)

Conquerage_34

Conquerage_35

Conquerage_36
(dsDNA partitioning protein)

Conquerage_37
(dsDNA partitioning protein)

Conquerage_38

Conquerage_39

Conquerage_40

Conquerage_41

Conquerage_42

Conquerage_43

Conquerage_44

Conquerage_45
(DNA polymerase I)

Conquerage_46

Conquerage_47

Conquerage_48

Conquerage_49
(thymidylate synthase)

Conquerage_50

Conquerage_51
(ribonucleotide reductase)

Conquerage_52

Conquerage_53

Conquerage_54

Conquerage_55

Conquerage_56
(metallophosphoesterase)

Conquerage_57

Conquerage_58
(glutaredoxin)

Conquerage_59
(DNA primase)

Conquerage_60
(DNA primase)

Conquerage_61

Conquerage_62
(endonuclease VII)

Conquerage_63

Conquerage_64

Conquerage_65

Conquerage_66

Conquerage_67

Conquerage_68

Conquerage_69
(dsDNA helicase)

Conquerage_70

Conquerage_71

Conquerage_72

Conquerage_73
(exonuclease)

Conquerage_74
(repressor)

Conquerage_75
(immunity repressor)

Conquerage_76

Conquerage_77

Conquerage_78

Conquerage_79

Conquerage_80

Conquerage_81

Conquerage_82
(DNA methylase)

Conquerage_83
(DNA methylase)

Conquerage_84

Conquerage_85

Conquerage_86

Conquerage_87

Conquerage_88

Conquerage_89

Conquerage_90

Conquerage_91

Conquerage_92

Conquerage_93

Conquerage_94

Conquerage_95

Conquerage_96

Conquerage_97

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG872835
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.7 x 10 ^12
Date of SEA Lysate Titering Oct 23, 2015
Pitt Freezer Box# 22
Pitt Freezer Box Grid# C4
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload