| Detailed Information for Phage Orange |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ryan Matthiessen |
| Year Found | 2018 |
| Location Found | Bethlehem, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.6045 N, 75.3749 W Map |
| Discovery Notes | Collected on February 5th, 2018 around 4:45PM behind Dravo House Residence Hall under one of the windows of the D2 hall at Lehigh University. Clear sky conditions but the ground had two to five centimeters of snow on the ground. Dug about 7.5 centimeters below the soil surface for soil collection.
|
| Naming Notes | It's not my favorite color. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 6, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1564 |
| Genome length (bp) | 52147 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.6% |
| Sequencing Notes | About 38% of reads in this genome indicate a 1117-bp deletion from 41805 to 42921, inclusive. The presumption is that it's a mixed population. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A11 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotation Status | In GenBank |
| Plaque Notes | Plaques had an average size of less than 0.5mm. The plaques were clear with no turbid ring or unique distinction around the plaques.
|
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524507 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1e11 |
| Pitt Freezer Box# |
84 |
| Pitt Freezer Box Grid# |
F6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
