| Detailed Information for Phage JSwag |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Alexandria Wojtak and Katelyn Chester |
| Year Found | 2015 |
| Location Found | Waterford, WI United States |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 42.790736 N, 88.229188 W Map |
| Discovery Notes | The soil was dry, loose, and some of the soil was clumped together. The soil was collected from a depth of around 152.4 mm. It was collected from a garden that is fertilized regularly with llama beans and compost. |
| Naming Notes | The name of this phage came from the biology teacher that Alexandria had in high school. He helped Alexandria become interested in biology as a major and helped to nurture a love of science in Alexandria. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 17, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2276 |
| Genome length (bp) | 52726 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGTGGTTA |
| GC Content | 61.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A15 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotation Status | In GenBank |
| Plaque Notes | The plagues were on the smaller side of average. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 3 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX557280 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.3*10^8 |
| Date of SEA Lysate Titering |
Oct 14, 2015 |
| Pitt Freezer Box# |
24 |
| Pitt Freezer Box Grid# |
C3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
