Detailed Information for Phage JSwag |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Found By | Alexandria Wojtak and Katelyn Chester |
Year Found | 2015 |
Location Found | Waterford, WI USA |
Finding Institution | University of Wisconsin-River Falls |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 25°C |
GPS Coordinates | 42.790736 N, 88.229188 W Map |
Discovery Notes | The soil was dry, loose, and some of the soil was clumped together. The soil was collected from a depth of around 152.4 mm. It was collected from a garden that is fertilized regularly with llama beans and compost. |
Naming Notes | The name of this phage came from the biology teacher that Alexandria had in high school. He helped Alexandria become interested in biology as a major and helped to nurture a love of science in Alexandria. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Feb 17, 2016 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 2276 |
Genome length (bp) | 52726 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGGTGGTTA |
GC Content | 61.9% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A15 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | The plagues were on the smaller side of average. |
Morphotype | Siphoviridae |
Number of Genes | 101 |
Number of tRNAs | 3 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KX557280 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
3.3*10^8 |
Date of SEA Lysate Titering |
Oct 14, 2015 |
Pitt Freezer Box# |
24 |
Pitt Freezer Box Grid# |
C3 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |