Mycobacterium phage Herbertwm
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Detailed Information for Phage Herbertwm
Discovery Information
Isolation HostMycobacterium aichiense ATCC 27280
Found ByTaiana James
Year Found2018
Location FoundWilliamsburg, VA USA
Finding InstitutionCollege of William & Mary
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature25°C
GPS Coordinates37.2734 N, 76.7178 W Map
Discovery NotesI got a plaque from my second soil sample enrichment (9/27/18). My soil came from the construction site near the student health center. From 22 students multiple (five) attempts each, HerbertWM was the only phage isolated. The titer remained very low and DNA was virtually impossible to obtain.
Sequencing Information
Sequencing Complete?No
Date Sequencing CompletedFeb 2, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage147
Genome length (bp)11104
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTCGGTTA
GC Content63.4%
Fasta file available?No
Characterization
ClusterSingleton
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotation StatusNot sequenced
Plaque NotesLarge, clear plaques.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload