Detailed Information for Phage Hoot |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Jon Thompson, Dr. Christy Strong |
Year Found | 2020 |
Location Found | Las Vegas, NV United States |
Finding Institution | University of Nevada Las Vegas |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 20°C |
GPS Coordinates | 36.110009 N, 115.142738 W Map |
Discovery Notes | The phage originated from a dark brown and coarse soil sample that was extracted from a potted plant with an unidentified red flower.
Time and Date of Extraction: 13:30 | 09/15/2020 Temperature of Area of Extraction: Room temperature (approximately 20°C). |
Naming Notes | Plaque morphology resembled owl eyes, especially when two plaques are directly adjacent to each other. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 22, 2021 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 236 |
Genome length (bp) | 50006 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGTCGGTTA |
GC Content | 61.5% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A6 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | University of Nevada Las Vegas |
Annotation Status | In GenBank |
Plaque Notes | -Circular clearing with a cloudy halo surrounding the center -Highly turbid -Approx. 7 mm diameter |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MZ171376 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
120 |
Pitt Freezer Box Grid# |
H3 |
Available Files |
Plaque Picture | Download |
GenBank File for Phamerator | Download |