| Detailed Information for Phage Hoot |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jon Thompson, Dr. Christy Strong |
| Year Found | 2020 |
| Location Found | Las Vegas, NV United States |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 20°C |
| GPS Coordinates | 36.110009 N, 115.142738 W Map |
| Discovery Notes | The phage originated from a dark brown and coarse soil sample that was extracted from a potted plant with an unidentified red flower.
Time and Date of Extraction: 13:30 | 09/15/2020
Temperature of Area of Extraction: Room temperature (approximately 20°C). |
| Naming Notes | Plaque morphology resembled owl eyes, especially when two plaques are directly adjacent to each other. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 236 |
| Genome length (bp) | 50006 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | In GenBank |
| Plaque Notes | -Circular clearing with a cloudy halo surrounding the center
-Highly turbid
-Approx. 7 mm diameter |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ171376 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
120 |
| Pitt Freezer Box Grid# |
H3 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
