The Actinobacteriophage Database

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Detailed Information for Phage Druantia
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Jacky Reny
Year Found	2016
Location Found	Bensalem, PA USA
Finding Institution	University of the Sciences in Philadelphia
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	27°C
GPS Coordinates	40.117291 N, 74.930833 W Map
Discovery Notes	The Soil was found in a location with dense pine tree population. The ground was flat with little to no inclination. The soil is damp with saturation from water. The temperature was 27°C at time of collection. The soil sample was collected near roots and veins of the tree. The soil sample was collected approximately 2 cm under ground level. The soil was littered with other organic materials. The date of collection was 8/22.
Naming Notes	In the Celtic mythology, the goddess of trees and knowledge is Druantia. As the sample was originally discovered in the forest and the exploration to gain knowledge was the driving force of SEA-PHAGES, the name seemed fitting.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 5, 2017
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	1828
Genome length (bp)	51343
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGCCGGTAA
GC Content	63.8%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	A
Subcluster	A4
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Abrogate (A1) Acme (A1) Adahisdi (A1) Aeneas (A1) AFIS (A1) Ajay (A1) Alsfro (A1) Alvin (A1) Anglerfish (A1) Arcanine (A1) Arlo (A1) Atkinbua (A1) BaconJack (A1) Barriga (A1) Beatrix (A1) BeesKnees (A1) Bethlehem (A1) Big3 (A1) Bigfoot (A1) BigMau (A1) BigPaolini (A1) BillKnuckles (A1) Bircsak (A1) Blue (A1) Bob3 (A1) Bones (A1) BPBiebs31 (A1) Briton15 (A1) Bruns (A1) Buttons (A1) Bxb1 (A1) CactusRose (A1) Carlyle (A1) ConceptII (A1) Corvo (A1) Crispicous1 (A1) Cueylyss (A1) DD5 (A1) Doom (A1) Dreamboat (A1) DreamCatcher (A1) DrFeelGood (A1) Dulcie (A1) Dynamix (A1) Edtherson (A1) Euphoria (A1) Fajezeel (A1) Fascinus (A1) Fenn (A1) Forsytheast (A1) Francis47 (A1) Froghopper (A1) Fushigi (A1) GageAP (A1) Gompeii16 (A1) Graduation (A1) Greg (A1) HanShotFirst (A1) HarryOW (A1) HermioneGrange (A1) Homines (A1) Hope4ever (A1) Ichabod (A1) ILeeKay (A1) JackSparrow (A1) Jasper (A1) JC27 (A1) Kanely (A1) KBG (A1) Killigrew (A1) KSSJEB (A1) Kugel (A1) Kykar (A1) KyMonks1A (A1) Lamina13 (A1) Lesedi (A1) Lockley (A1) Lopton (A1) Magnar (A1) Magnito (A1) Makemake (A1) Manatee (A1) Marcell (A1) Maroc7 (A1) McGuire (A1) MetalQZJ (A1) Michley (A1) Moose (A1) MPlant7149 (A1) MrGordo (A1) Mryolo (A1) Mule (A1) Museum (A1) Naira (A1) NEHalo (A1) Nerujay (A1) Nhonho (A1) Niza (A1) Oogway (A1) PacerPaul (A1) Papez (A1) Paphu (A1) Pari (A1) PattyP (A1) Pepe (A1) Perseus (A1) Petp2012 (A1) Petruchio (A1) PherrisBueller (A1) PhrostyMug (A1) PinkPlastic (A1) Pinto (A1) Pippin (A1) Pita2 (A1) PSullivan (A1) Rajelicia (A1) Rhynn (A1) RidgeCB (A1) Ringer (A1) Rohr (A1) Rufus (A1) Ruotula (A1) Rutherferd (A1) Sagefire (A1) SarFire (A1) Scowl (A1) Seabiscuit (A1) Sibs6 (A1) SkiPole (A1) Smairt (A1) Smeagol (A1) Snazzy (A1) Solon (A1) SpikeBT (A1) Squee (A1) StrongArm (A1) Sumter (A1) SwissCheese (A1) Switzer (A1) Target (A1) Tasp14 (A1) TheloniousMonk (A1) Thor (A1) Traft412 (A1) Treddle (A1) Tripl3t (A1) Trouble (A1) Turj99 (A1) U2 (A1) Violet (A1) Watermelon (A1) Wheeler (A1) Wilkins (A1) Zeeculate (A1) Zephyr (A1) Zeuska (A1) Chupacabra (A10) Goose (A10) KittenMittens (A10) Kristoff (A10) OKCentral2016 (A10) Rebeuca (A10) RhynO (A10) Severus (A10) Trike (A10) Twister (A10) WalterMcMickey (A10) Aneem (A11) Bachome (A11) Bowtie (A11) Bud (A11) Ebony (A11) Et2Brutus (A11) Fibonacci (A11) Flaverint (A11) Hutc2 (A11) Jabith (A11) Joselito (A11) Lucivia (A11) Mabel (A11) Mulciber (A11) Munch (A11) Orange (A11) Salz (A11) Snape (A11) DarthPhader (A12) Pharaoh (A12) Refuge (A12) Steamy (A12) Phlei (A13) Jeeves (A14) Luchador (A14) Anon (A15) JSwag (A15) KatherineG (A15) LastResort (A15) MinecraftSteve (A15) Nedarya (A15) ReMo (A15) Remus (A15) Rosalind (A15) ShayRa (A15) Soups (A15) Strosahl (A15) Waits (A15) EagleEye (A16) Lucyedi (A16) 40AC (A17) MyraDee (A18) Kimona (A19) 20ES (A2) AbbyPaige (A2) Acolyte (A2) Adzzy (A2) AN3 (A2) AN9 (A2) ANI8 (A2) AnnaL29 (A2) Anselm (A2) ArcherNM (A2) Bactobuster (A2) Baehexic (A2) Benvolio (A2) BiancaTri92 (A2) BobSwaget (A2) Bugsy (A2) Centaur (A2) Changeling (A2) Che12 (A2) ChipMunk (A2) CRB1 (A2) Crucio (A2) D29 (A2) D32 (A2) Dalmatian (A2) DBQu4n (A2) Drake55 (A2) DudeLittle (A2) Echild (A2) Equemioh13 (A2) EvilGenius (A2) Fameo (A2) FiringLine (A2) First (A2) Flare16 (A2) GaugeLDP (A2) Georgie2 (A2) Heffalump (A2) Herbertwm (A2) IronMan (A2) Iwokeuplikedis (A2) Jaan (A2) Jerm (A2) JoshKayV (A2) Journey13 (A2) Jsquared (A2) Kalpine (A2) Kerberos (A2) L5 (A2) LadyBird (A2) Larenn (A2) Leogania (A2) LilTurb (A2) Lokk (A2) Loser (A2) Malec (A2) Miko (A2) MissWhite (A2) Naji (A2) NaSiaTalie (A2) NicoleTera (A2) NothingSpecial (A2) Odin (A2) Ph8s (A2) Phaded (A2) Piro94 (A2) Pomar16 (A2) Power (A2) Pukovnik (A2) QueenB2 (A2) QueenBeesly (A2) Rachaly (A2) RedRock (A2) Retro23 (A2) SemperFi (A2) Serenity (A2) SnapTap (A2) StarStuff (A2) Superchunk (A2) SweetiePie (A2) TipsytheTRex (A2) Tomathan (A2) Trixie (A2) Turbido (A2) Updawg (A2) VA6 (A2) VC3 (A2) Whabigail7 (A2) WideWale (A2) XianYue (A2) Anthony (A20) ACFishhook (A3) Aglet (A3) AgronaGT15 (A3) Anubis (A3) AugsMagnumOpus (A3) BabyRay (A3) Beauxregard13 (A3) BreSam8 (A3) BuzzBuzz (A3) Bxz2 (A3) DaHudson (A3) Daishi (A3) Dieselweasel (A3) EpicPhail (A3) Farber (A3) Fernando (A3) Fred313 (A3) Gemma (A3) GingkoMaracino (A3) Groupthink (A3) Grum1 (A3) GtownJaz (A3) Heathen (A3) HelDan (A3) Heliosoles (A3) Hercules11 (A3) Hookmount (A3) Idleandcovert (A3) Isca (A3) JenCasNa (A3) JeppNRM (A3) JF2 (A3) JF4 (A3) JHC117 (A3) Jobu08 (A3) KADY (A3) Kalb97 (A3) Kalnoky (A3) Lambert1 (A3) Lilith (A3) Louie6 (A3) LugYA (A3) MA5 (A3) MadMarie (A3) Mainiac (A3) Malinsilva (A3) Margo (A3) Marie (A3) Marius (A3) MarQuardt (A3) Methuselah (A3) Microwolf (A3) Misomonster (A3) MK4 (A3) MoneyMay (A3) MuchMore (A3) Noella (A3) Norbert (A3) OlanP (A3) Ollie (A3) P28Green (A3) Panamaxus (A3) Penny1 (A3) PGHhamlin (A3) Phantastic (A3) PhishRPhriends (A3) Phoxy (A3) Phranny (A3) Pistachio (A3) Pocahontas (A3) Popcicle (A3) PotatoSplit (A3) Puppy (A3) PurpleHaze (A3) QuinnKiro (A3) Reba (A3) Rockstar (A3) Sabia (A3) Sabinator (A3) SoilDragon (A3) Soshari (A3) Spike509 (A3) Stagni (A3) StepMih (A3) Taurus (A3) Texage (A3) Tiffany (A3) TNguyen7 (A3) Todacoro (A3) Veracruz (A3) Vix (A3) Watson (A3) Wooldri (A3) Zetzy (A3) AbbysRanger (A4) Abdiel (A4) Achebe (A4) Albee (A4) Annyong (A4) Arturo (A4) AvatarAhPeg (A4) Avle17 (A4) Baby16 (A4) Backyardigan (A4) Badger (A4) Bartimeaus (A4) BellusTerra (A4) Blackmoor (A4) Bombshell (A4) Broseidon (A4) Bruiser (A4) BubbleTrouble (A4) Bumblebee11 (A4) Burger (A4) Caelakin (A4) Camperdownii (A4) CentreCat (A4) Cerulean (A4) ChampagnePapi (A4) Cici (A4) Cindaradix (A4) Cintron (A4) Citius (A4) Clarenza (A4) Cocoaberry (A4) Commander (A4) Connomayer (A4) Datway (A4) Dhanush (A4) DirtyDunning (A4) Druantia (A4) Eagle (A4) Eapen (A4) Eris (A4) Eros (A4) Florean (A4) Flux (A4) Funston (A4) Gadost (A4) HamSlice (A4) Holli (A4) Houdini22 (A4) ICleared (A4) Iracema64 (A4) Jaykayelowell (A4) JetBlade (A4) JoongJeon (A4) Kampy (A4) Katalie136 (A4) KFPoly (A4) Kingmustik0402 (A4) Kratark (A4) Kyee (A4) Lemur (A4) LeoAvram (A4) LHTSCC (A4) LittleB (A4) LittleGuy (A4) Lorenzo (A4) Maverick (A4) Mazhar510 (A4) Medusa (A4) MeeZee (A4) Melvin (A4) Millski (A4) Miramae (A4) Morpher26 (A4) Morrow (A4) Mundrea (A4) Nebs (A4) Nemo27 (A4) Noelle (A4) NorthStar (A4) NotAPhaseMom (A4) Nyxis (A4) Obama12 (A4) Ohfah (A4) Palestino (A4) Peaches (A4) PeterPeter (A4) Phacado (A4) Phighter1804 (A4) Phontbonne (A4) Pipcraft (A4) Relief (A4) Romney (A4) Roosevelt (A4) Ruin (A4) Sabertooth (A4) Shaka (A4) SheldonCooper (A4) Skipitt (A4) Stasia (A4) Thanksgivukkah (A4) TinaFeyge (A4) Tinybot (A4) TiroTheta9 (A4) TygerBlood (A4) Wander (A4) Wilbur (A4) Wile (A4) Wizard007 (A4) Xena (A4) AgentM (A5) Airmid (A5) Aragog (A5) Archetta (A5) Benedict (A5) Bluefalcon (A5) Chadwick (A5) Conspiracy (A5) Coog (A5) Cuco (A5) Dublin (A5) ElTiger69 (A5) ForGetIt (A5) George (A5) HuhtaEnerson15 (A5) Jabiru (A5) Jovo (A5) Lev2 (A5) LittleCherry (A5) MarysWell (A5) Midas2 (A5) Naca (A5) PetterN (A5) Phlorence (A5) Scorpia (A5) Swirley (A5) Tarynearal (A5) Theia (A5) Tiger (A5) UnionJack (A5) Zolita (A5) Artemis2UCLA (A6) Blinn1 (A6) Blue7 (A6) CloudWang3 (A6) DaVinci (A6) EricB (A6) Gladiator (A6) GreedyLawyer (A6) Gruunaga (A6) Hammer (A6) Hexamo (A6) Isiphiwo (A6) Jeffabunny (A6) JewelBug (A6) Jordennis (A6) Kazan (A6) Kipper29 (A6) Koko (A6) McFly (A6) Pmask (A6) Priamo (A6) SuperAwesome (A6) ToneTone (A6) VohminGhazi (A6) Wiks (A6) WunderPhul (A6) Yokurt (A6) Zaka (A6) Zulu (A6) HINdeR (A7) Sheen (A7) Timshel (A7) Astro (A8) Dixon (A8) Expelliarmus (A8) NearlyHeadless (A8) Roary (A8) Saintus (A8) Smeadley (A8) Stephig9 (A8) Aliter (A9) Alma (A9) Arissanae (A9) Beemo (A9) BogosyJay (A9) Catalina (A9) Charm (A9) Conquerage (A9) DreamTeam1 (A9) Eidsmoe (A9) Elephantoon (A9) ExplosioNervosa (A9) HortumSL17 (A9) Keziacharles14 (A9) LoneWolf (A9) Maminiaina (A9) Myxus (A9) PackMan (A9) Phaeder (A9) Phonnegut (A9) Pioneer (A9) Priya (A9) Qobbit (A9) Rahalelujah (A9) Scherzo (A9) SheaKeira (A9) Toaka (A9) Tubs (A9) Ugenie5 (A9) Vanisoa (A9) Yecey3 (A9)
Annotating Institution	University of the Sciences in Philadelphia
Annotation Status	In GenBank
Plaque Notes	The plaques were turbid and the perimeter of the plaque looked consistent the other plaques. Some of the plaques were bigger than others, which initially indicated the presence of multiple phage. However, after several repeated isolations, the same dissimilar sizes appeared, which lead to the hypothesis that this specific phage is density dependent. The diameter of the plaques ranged from 3mm to 4mm.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Druantia_1 (HNH endonuclease) Druantia_2 Druantia_3 Druantia_4 Druantia_5 Druantia_6 Druantia_7 Druantia_8 (lysin A) Druantia_9 (holin) Druantia_10 (lysin B) Druantia_11 (terminase) Druantia_12 (portal protein) Druantia_13 (capsid maturation protease) Druantia_14 (scaffolding protein) Druantia_15 (major capsid protein) Druantia_16 Druantia_17 (head-to-tail adaptor) Druantia_18 Druantia_19 (head-to-tail stopper) Druantia_20 Druantia_21 (tail terminator) Druantia_22 (major tail protein) Druantia_23 (tail assembly chaperone) Druantia_24 (tail assembly chaperone) Druantia_25 (tape measure protein) Druantia_26 (minor tail protein) Druantia_27 (minor tail protein) Druantia_28 (minor tail protein) Druantia_29 (minor tail protein) Druantia_30 (minor tail protein) Druantia_31 (minor tail protein) Druantia_32 Druantia_33 (integrase) Druantia_34 Druantia_35 (deoxycytidylate deaminase) Druantia_36 Druantia_37 Druantia_38 Druantia_39 Druantia_40 (helix-turn-helix DNA binding domain protein) Druantia_41 Druantia_42 Druantia_43 Druantia_44 Druantia_45 (DNA polymerase I) Druantia_46 (helix-turn-helix DNA binding domain protein) Druantia_47 Druantia_48 (ThyX-like thymidylate synthase) Druantia_49 Druantia_50 (ribonucleotide reductase) Druantia_51 (helix-turn-helix DNA binding domain protein) Druantia_52 (metallophosphoesterase) Druantia_53 Druantia_54 Druantia_55 (DNA primase) Druantia_56 (DNA primase) Druantia_57 Druantia_58 (endonuclease VII) Druantia_59 (hydrolase) Druantia_60 Druantia_61 Druantia_62 (phosphoribosyl transferase) Druantia_63 (DnaB-like dsDNA helicase) Druantia_64 Druantia_65 Druantia_66 Druantia_67 (Cas4 family exonuclease) Druantia_68 Druantia_69 (immunity repressor) Druantia_70 Druantia_71 Druantia_72 Druantia_73 Druantia_74 Druantia_75 Druantia_76 Druantia_77 (DNA methylase) Druantia_78 (DNA methylase) Druantia_79 (SprT-like protease) Druantia_80 Druantia_81 Druantia_82 Druantia_83 Druantia_84 Druantia_85 Druantia_86 Druantia_87
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MH536817
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	34
Pitt Freezer Box Grid#	E2
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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