| Detailed Information for Phage Refuge |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Brittnie Hodsdon, Hateya Levesque, Billy & Jon |
| Year Found | 2016 |
| Location Found | Saint John, ME USA |
| Finding Institution | University of Maine, Fort Kent |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 47.222278 N, 68.761303 W Map |
| Discovery Notes | Discovered near Jalbert crossing along the St. John river. Found in silty sediment, oily sheen and saturated with water. |
| Naming Notes | The name of this refuge, due to inconclusive results from other soil samples. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1607 |
| Genome length (bp) | 53594 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGACGTAA |
| GC Content | 63.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A12 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotation Status | In GenBank |
| Plaque Notes | Extracted a phage from a soil sample, obtained a single plaque, picked this plaque and did serial dilutions and was able to get a series of plates. Started with web plate and had less plaques with each plate. Amplifid phage stock to get the webbed plate for titering. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK494113 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.7 x10^8 pfu/mL |
| Date of SEA Lysate Titering |
Sep 1, 2017 |
| Pitt Freezer Box# |
46 |
| Pitt Freezer Box Grid# |
D1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
