| Detailed Information for Phage Rohr |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rahul Ramaswamy and Hannah Olsen |
| Year Found | 2014 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.647706 N, 90.30637 W Map |
| Discovery Notes | Discovered on a small hill near Duncker Hall on the Washington University in St. Louis Danforth campus.
6'' deep into the soil.
The soil was lightly colored and slightly moist.
The weather was temperate - sunny and 70. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 25, 2018 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 207 |
| Genome length (bp) | 53483 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Rohr is most likely lytic due to the consistency of clear, circular plaques. |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are consistently circular and clear but vary in radius size from 0.5mm-3.0mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH450130 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
3.28 x 10^9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
