The Actinobacteriophage Database

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Detailed Information for Phage Badger
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Stevie Hoyng
Year Found	2014
Location Found	Bowling Green, KY USA
Finding Institution	Western Kentucky University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	36.988611 N, 86.448333 W Map
Discovery Notes	The soil came from my backyard from underneath a bird bath. The bird bath is heavily used by birds, as well as my neighbor’s cat. The soil had many different types of insects living it. The soil was very moist. The bird bath is located about 50 feet from a road that is heavily traveled by cars and people. Around the bird bath is plenty of grass and about two feet away is a row of day lilies. There is a lilac bush, Australian Pine Tree, and a Magnolia Tree in the yard.
Naming Notes	I am from Pittsburgh and I am a huge hockey fan. My phage is named after "Badger Bob" Johnson who led the Pittsburgh Penguins to their first Stanley Cup Championship in 1991.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 22, 2014
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	274
Genome length (bp)	51274
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGCCGGTTA
GC Content	63.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	A
Subcluster	A4
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Abrogate (A1) Acme (A1) Adahisdi (A1) Aeneas (A1) AFIS (A1) Ajay (A1) Alsfro (A1) Alvin (A1) Anglerfish (A1) Arcanine (A1) Arlo (A1) Atkinbua (A1) BaconJack (A1) Barriga (A1) Beatrix (A1) BeesKnees (A1) Bethlehem (A1) Big3 (A1) Bigfoot (A1) BigMau (A1) BigPaolini (A1) BillKnuckles (A1) Bircsak (A1) Blue (A1) Bob3 (A1) Bones (A1) BPBiebs31 (A1) Briton15 (A1) Bruns (A1) Buttons (A1) Bxb1 (A1) CactusRose (A1) Carlyle (A1) ConceptII (A1) Corvo (A1) Crispicous1 (A1) Cueylyss (A1) DD5 (A1) Doom (A1) Dreamboat (A1) DreamCatcher (A1) DrFeelGood (A1) Dulcie (A1) Dynamix (A1) Edtherson (A1) Euphoria (A1) Fajezeel (A1) Fascinus (A1) Fenn (A1) Forsytheast (A1) Francis47 (A1) Froghopper (A1) Fushigi (A1) GageAP (A1) Gompeii16 (A1) Graduation (A1) Greg (A1) HanShotFirst (A1) HarryOW (A1) HermioneGrange (A1) Homines (A1) Hope4ever (A1) Ichabod (A1) ILeeKay (A1) JackSparrow (A1) Jasper (A1) JC27 (A1) Kanely (A1) KBG (A1) Killigrew (A1) KSSJEB (A1) Kugel (A1) Kykar (A1) KyMonks1A (A1) Lamina13 (A1) Lesedi (A1) Lockley (A1) Lopton (A1) Magnar (A1) Magnito (A1) Makemake (A1) Manatee (A1) Marcell (A1) Maroc7 (A1) McGuire (A1) MetalQZJ (A1) Michley (A1) Moose (A1) MPlant7149 (A1) MrGordo (A1) Mryolo (A1) Mule (A1) Museum (A1) Naira (A1) NEHalo (A1) Nerujay (A1) Nhonho (A1) Niza (A1) Oogway (A1) PacerPaul (A1) Papez (A1) Paphu (A1) Pari (A1) PattyP (A1) Pepe (A1) Perseus (A1) Petp2012 (A1) Petruchio (A1) PherrisBueller (A1) PhrostyMug (A1) PinkPlastic (A1) Pinto (A1) Pippin (A1) Pita2 (A1) PSullivan (A1) Rajelicia (A1) Rhynn (A1) RidgeCB (A1) Ringer (A1) Rohr (A1) Rufus (A1) Ruotula (A1) Rutherferd (A1) Sagefire (A1) SarFire (A1) Scowl (A1) Seabiscuit (A1) Sibs6 (A1) SkiPole (A1) Smairt (A1) Smeagol (A1) Snazzy (A1) Solon (A1) SpikeBT (A1) Squee (A1) StrongArm (A1) Sumter (A1) SwissCheese (A1) Switzer (A1) Target (A1) Tasp14 (A1) TheloniousMonk (A1) Thor (A1) Traft412 (A1) Treddle (A1) Tripl3t (A1) Trouble (A1) Turj99 (A1) U2 (A1) Violet (A1) Watermelon (A1) Wheeler (A1) Wilkins (A1) Zeeculate (A1) Zephyr (A1) Zeuska (A1) Chupacabra (A10) Drake94 (A10) Goose (A10) KittenMittens (A10) Kristoff (A10) OKCentral2016 (A10) Rebeuca (A10) RhynO (A10) Severus (A10) Trike (A10) Twister (A10) WalterMcMickey (A10) Aneem (A11) Bachome (A11) Bowtie (A11) Bud (A11) Ebony (A11) Et2Brutus (A11) Fibonacci (A11) Flaverint (A11) Hutc2 (A11) Jabith (A11) Joselito (A11) Lucivia (A11) Mabel (A11) Mulciber (A11) Munch (A11) Orange (A11) Salz (A11) Snape (A11) DarthPhader (A12) Pharaoh (A12) Refuge (A12) Steamy (A12) Phlei (A13) Jeeves (A14) Luchador (A14) Anon (A15) JSwag (A15) KatherineG (A15) LastResort (A15) MinecraftSteve (A15) Nedarya (A15) ReMo (A15) Remus (A15) Rosalind (A15) ShayRa (A15) Soups (A15) Strosahl (A15) Waits (A15) EagleEye (A16) Lucyedi (A16) 40AC (A17) MyraDee (A18) Kimona (A19) 20ES (A2) AbbyPaige (A2) Acolyte (A2) Adzzy (A2) AN3 (A2) AN9 (A2) ANI8 (A2) AnnaL29 (A2) Anselm (A2) ArcherNM (A2) Bactobuster (A2) Baehexic (A2) Benvolio (A2) BiancaTri92 (A2) BobSwaget (A2) Bugsy (A2) Centaur (A2) Changeling (A2) Che12 (A2) ChipMunk (A2) CRB1 (A2) Crucio (A2) D29 (A2) D32 (A2) Dalmatian (A2) DBQu4n (A2) Drake55 (A2) DudeLittle (A2) Echild (A2) Equemioh13 (A2) EvilGenius (A2) Fameo (A2) FiringLine (A2) First (A2) Flare16 (A2) GaugeLDP (A2) Georgie2 (A2) Heffalump (A2) Herbertwm (A2) IronMan (A2) Iwokeuplikedis (A2) Jaan (A2) Jerm (A2) JoshKayV (A2) Journey13 (A2) Jsquared (A2) Kalpine (A2) Kerberos (A2) L5 (A2) LadyBird (A2) Larenn (A2) Leogania (A2) LilTurb (A2) Lokk (A2) Loser (A2) Malec (A2) Miko (A2) MissWhite (A2) Naji (A2) NaSiaTalie (A2) NicoleTera (A2) NothingSpecial (A2) Odin (A2) Ph8s (A2) Phaded (A2) Piro94 (A2) Pomar16 (A2) Power (A2) Pukovnik (A2) QueenB2 (A2) QueenBeesly (A2) Rachaly (A2) RedRock (A2) Retro23 (A2) SemperFi (A2) Serenity (A2) SnapTap (A2) StarStuff (A2) Superchunk (A2) SweetiePie (A2) TipsytheTRex (A2) Tomathan (A2) Trixie (A2) Turbido (A2) Updawg (A2) VA6 (A2) VC3 (A2) Whabigail7 (A2) WideWale (A2) XianYue (A2) Anthony (A20) ACFishhook (A3) Aglet (A3) AgronaGT15 (A3) Anubis (A3) AugsMagnumOpus (A3) BabyRay (A3) Beauxregard13 (A3) BreSam8 (A3) BuzzBuzz (A3) Bxz2 (A3) Caviar (A3) DaHudson (A3) Daishi (A3) Dieselweasel (A3) EpicPhail (A3) Farber (A3) Fernando (A3) Fred313 (A3) Gemma (A3) GingkoMaracino (A3) Groupthink (A3) Grub (A3) Grum1 (A3) GtownJaz (A3) Heathen (A3) HelDan (A3) Heliosoles (A3) Hercules11 (A3) Hookmount (A3) Idleandcovert (A3) Isca (A3) JenCasNa (A3) JeppNRM (A3) JF2 (A3) JF4 (A3) JHC117 (A3) Jobu08 (A3) KADY (A3) Kalb97 (A3) Kalnoky (A3) Lambert1 (A3) Lilith (A3) Louie6 (A3) LugYA (A3) MA5 (A3) MadMarie (A3) Mainiac (A3) Malinsilva (A3) Margo (A3) Marie (A3) Marius (A3) MarQuardt (A3) Methuselah (A3) Microwolf (A3) Misomonster (A3) MK4 (A3) MoneyMay (A3) MuchMore (A3) Noella (A3) Norbert (A3) OlanP (A3) Ollie (A3) P28Green (A3) Panamaxus (A3) Penny1 (A3) PGHhamlin (A3) Phantastic (A3) PhishRPhriends (A3) Phoxy (A3) Phranny (A3) Pistachio (A3) Pocahontas (A3) Popcicle (A3) PotatoSplit (A3) Puppy (A3) PurpleHaze (A3) QuinnKiro (A3) Reba (A3) Rockstar (A3) Sabia (A3) Sabinator (A3) SoilDragon (A3) Soshari (A3) Spike509 (A3) Stagni (A3) StepMih (A3) Taurus (A3) Texage (A3) Tiffany (A3) TNguyen7 (A3) Todacoro (A3) Veracruz (A3) Vix (A3) Watson (A3) Wooldri (A3) Zetzy (A3) AbbysRanger (A4) Abdiel (A4) Achebe (A4) Albee (A4) Annyong (A4) Arturo (A4) AvatarAhPeg (A4) Avle17 (A4) Baby16 (A4) Backyardigan (A4) Badger (A4) Bartimeaus (A4) BellusTerra (A4) Blackmoor (A4) Bombshell (A4) Broseidon (A4) Bruiser (A4) BubbleTrouble (A4) Bumblebee11 (A4) Burger (A4) Caelakin (A4) Camperdownii (A4) CentreCat (A4) Cerulean (A4) ChampagnePapi (A4) Cici (A4) Cindaradix (A4) Cintron (A4) Citius (A4) Clarenza (A4) Cocoaberry (A4) Commander (A4) Connomayer (A4) Datway (A4) Deano (A4) Dhanush (A4) DirtyDunning (A4) Druantia (A4) Eagle (A4) Eapen (A4) Eris (A4) Eros (A4) Florean (A4) Flux (A4) Funston (A4) Gadost (A4) HamSlice (A4) Holli (A4) Houdini22 (A4) ICleared (A4) Iracema64 (A4) Jaykayelowell (A4) JetBlade (A4) JoongJeon (A4) Kampy (A4) Katalie136 (A4) KFPoly (A4) Kingmustik0402 (A4) Kratark (A4) Kyee (A4) Lemur (A4) LeoAvram (A4) LHTSCC (A4) LittleB (A4) LittleGuy (A4) Lorenzo (A4) Maverick (A4) Mazhar510 (A4) Medusa (A4) MeeZee (A4) Melvin (A4) Millski (A4) Miramae (A4) Morpher26 (A4) Morrow (A4) Mundrea (A4) Nebs (A4) Nemo27 (A4) Noelle (A4) NorthStar (A4) NotAPhaseMom (A4) Nyxis (A4) Obama12 (A4) Ohfah (A4) Palestino (A4) Peaches (A4) PeterPeter (A4) Phacado (A4) Phighter1804 (A4) Phontbonne (A4) Pipcraft (A4) Polymorphads (A4) Relief (A4) Romney (A4) Roosevelt (A4) Ruin (A4) Sabertooth (A4) Scamp (A4) Shaka (A4) SheldonCooper (A4) Skipitt (A4) Stasia (A4) Thanksgivukkah (A4) TinaFeyge (A4) Tinybot (A4) TiroTheta9 (A4) TroyPia (A4) TygerBlood (A4) Wander (A4) Wilbur (A4) Wile (A4) Wizard007 (A4) Xena (A4) AgentM (A5) Airmid (A5) Aragog (A5) Archetta (A5) Benedict (A5) Bluefalcon (A5) Chadwick (A5) Conspiracy (A5) Coog (A5) Cuco (A5) Dublin (A5) ElTiger69 (A5) ForGetIt (A5) George (A5) HuhtaEnerson15 (A5) Jabiru (A5) Jovo (A5) Lev2 (A5) LittleCherry (A5) MarysWell (A5) Midas2 (A5) Naca (A5) PetterN (A5) Phlorence (A5) Scorpia (A5) Swirley (A5) Tarynearal (A5) Theia (A5) Tiger (A5) UnionJack (A5) Zolita (A5) Artemis2UCLA (A6) Blinn1 (A6) Blue7 (A6) CloudWang3 (A6) DaVinci (A6) EricB (A6) Gladiator (A6) GreedyLawyer (A6) Gruunaga (A6) Hammer (A6) Hexamo (A6) Isiphiwo (A6) Jeffabunny (A6) JewelBug (A6) Jordennis (A6) Kazan (A6) Kipper29 (A6) Koko (A6) McFly (A6) Pmask (A6) Priamo (A6) SuperAwesome (A6) ToneTone (A6) VohminGhazi (A6) Wiks (A6) WunderPhul (A6) Yokurt (A6) Zaka (A6) Zulu (A6) HINdeR (A7) Sheen (A7) Timshel (A7) Astro (A8) Dixon (A8) Expelliarmus (A8) NearlyHeadless (A8) Roary (A8) Saintus (A8) Smeadley (A8) Stephig9 (A8) Aliter (A9) Alma (A9) Arissanae (A9) Beemo (A9) BogosyJay (A9) Catalina (A9) Charm (A9) Conquerage (A9) DreamTeam1 (A9) Eidsmoe (A9) Elephantoon (A9) ExplosioNervosa (A9) HortumSL17 (A9) Keziacharles14 (A9) LoneWolf (A9) Maminiaina (A9) Myxus (A9) PackMan (A9) Phaeder (A9) Phonnegut (A9) Pioneer (A9) Priya (A9) Qobbit (A9) Rahalelujah (A9) Scherzo (A9) SheaKeira (A9) Toaka (A9) Tubs (A9) Ugenie5 (A9) Vanisoa (A9) Yecey3 (A9)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	The plaques have been bull eyes, they have been dark in the middle and clear on the edge. They have been about 1-3 mm in diameter. They are temperate.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Badger_1 (HNH endonuclease) Badger_2 Badger_3 Badger_4 (tail protein) Badger_5 Badger_6 Badger_7 Badger_8 (lysin A) Badger_9 (holin) Badger_10 (lysin B) Badger_11 (terminase) Badger_12 (portal protein) Badger_13 (capsid maturation protease) Badger_14 (scaffolding protein) Badger_15 (major capsid protein) Badger_16 Badger_17 (head-to-tail adaptor) Badger_18 Badger_19 (head-to-tail stopper) Badger_20 Badger_21 (tail terminator) Badger_22 (major tail protein) Badger_23 (tail assembly chaperone) Badger_24 (tail assembly chaperone) Badger_25 (tape measure protein) Badger_26 (minor tail protein) Badger_27 (minor tail protein) Badger_28 (minor tail protein) Badger_29 (minor tail protein) Badger_30 (minor tail protein) Badger_31 (minor tail protein) Badger_32 Badger_33 (integrase) Badger_34 Badger_35 (deoxycytidylate deaminase) Badger_36 Badger_37 (helix-turn-helix DNA binding domain protein) Badger_38 Badger_39 Badger_40 Badger_41 Badger_42 Badger_43 (DNA polymerase I) Badger_44 (HTH DNA binding domain protein) Badger_45 Badger_46 (thymidylate synthase) Badger_47 Badger_48 (ribonucleotide reductase) Badger_49 Badger_50 (esterase) Badger_51 Badger_52 Badger_53 (DNA primase) Badger_54 (DNA primase) Badger_55 (endonuclease VII) Badger_56 Badger_57 (hydrolase) Badger_58 Badger_59 Badger_60 (phosphoribosyltransferase) Badger_61 Badger_62 (dsDNA helicase) Badger_63 Badger_64 Badger_65 Badger_66 (Cas4 family exonuclease) Badger_67 Badger_68 (immunity repressor) Badger_69 Badger_70 Badger_71 Badger_72 Badger_73 Badger_74 (DNA methylase) Badger_75 (DNA methylase) Badger_76 (protease) Badger_77 Badger_78 Badger_79 Badger_80 Badger_81 Badger_82 Badger_83 Badger_84 Badger_85
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KX550442
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Designator	2014WEKUbadgerSH
SEA Lysate Titer	3.3 x 10^8
Date of SEA Lysate Titering	Oct 16, 2014
Pitt Freezer Box#	10
Pitt Freezer Box Grid#	C8
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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