| Detailed Information for Phage Miko |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Elma Herrera |
| Year Found | 2015 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 47.666456 N, 117.404914 W Map |
| Discovery Notes | The sample in which the phage was discovered came from Lake Arthur in Spokane, WA. |
| Mutants |
| Mutants of this Phage |
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 23, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3109 |
| Genome length (bp) | 51881 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.5% |
| Sequencing Notes | Sample contains a mixed population with approximately 25% of reads containing a 153 bp deletion of consensus 24718-24870 bp |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | QC Complete |
| Plaque Notes | All plaques are similar in size, having a diameter of approximately 3mm. They are also lytic meaning that the plaques are clear and easy to see. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.3 * 10^9 |
| Date of SEA Lysate Titering |
Jun 9, 2015 |
| Pitt Freezer Box# |
30 |
| Pitt Freezer Box Grid# |
A7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
