| Detailed Information for Phage McFly |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Seth Ayres |
| Year Found | 2012 |
| Location Found | Hampden-Sydney, VA USA |
| Finding Institution | Hampden-Sydney College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.240169 N, 78.460983 W Map |
| Discovery Notes | The soil sample that this phage was isolated from was collected from a depth of approximately 2.5 centimeters. The soil was collected from underneath of a hedgerow behind the Venable Hall dormitory on the Hampden-Sydney College campus. |
| Naming Notes | This phage is named after the main character in the "Back to the Future" film series. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 800 |
| Genome length (bp) | 52502 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU695582 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
28 |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
