Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Tyler Yates and Piper Replogle |
Year Found | 2013 |
Location Found | Boulder, CO USA |
Finding Institution | University of Colorado at Boulder |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 40.007444 N, 105.272029 W Map |
Discovery Notes | Flower bed, damp soil 09/16/2013 9:40 AM 14° C |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 10, 2014 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Ion Torrent Sequencing |
Approximate Shotgun Coverage | 886 |
Sanger Finishing Reactions | 8 |
Genome length (bp) | 50841 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGGTGGTAA |
GC Content | 64.1% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A3 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | University of Colorado at Boulder |
Annotation Status | In GenBank |
Plaque Notes | Approximately 2 mm, turbid throughout, 48 hour incubation, 37° C |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MH020238 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
2013UCBOLilithRY |
Date of SEA Lysate Titering |
Nov 6, 2013 |
Pitt Freezer Box# |
3 |
Pitt Freezer Box Grid# |
C4 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |