| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tyler Yates and Piper Replogle |
| Year Found | 2013 |
| Location Found | Boulder, CO USA |
| Finding Institution | University of Colorado at Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.007444 N, 105.272029 W Map |
| Discovery Notes | Flower bed, damp soil
09/16/2013
9:40 AM
14° C |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 886 |
| Sanger Finishing Reactions | 8 |
| Genome length (bp) | 50841 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGTGGTAA |
| GC Content | 64.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Colorado at Boulder |
| Annotation Status | In GenBank |
| Plaque Notes | Approximately 2 mm, turbid throughout, 48 hour incubation, 37° C |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH020238 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2013UCBOLilithRY |
| Date of SEA Lysate Titering |
Nov 6, 2013 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
C4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
