Detailed Information for Phage Loser |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Former names | L0S3R |
Found By | Lissenya B. Argueta |
Year Found | 2009 |
Location Found | Provo Canyon, UT USA |
Finding Institution | Brigham Young University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 40.32945 N, 111.621583 W Map |
Discovery Notes | Found on 1 Sep. 2009 around 2:00 p.m. on a riverbank about two inches into the soil. The approx. temp. was 65°F. Soil was loose and very moist. Tree roots and dead bugs were also around the sample. |
Naming Notes | Frustration with the isolation process. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Apr 18, 2012 |
Sequencing Facility | BYU DNA Sequencing Center |
Shotgun Sequencing Method | 454 Pyrosequencing |
Approximate Shotgun Coverage | 224 |
Genome length (bp) | 53486 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGTCGGTAA |
GC Content | 64.5% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A2 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Small and turbid, approx. 1mm in diameter after 48 hours. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KU761558 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
2009BRYO10s3rLA |
SEA Lysate Titer |
2e8 |
Date of SEA Lysate Titering |
Dec 22, 2010 |
Pitt Freezer Box# |
11 |
Pitt Freezer Box Grid# |
67 |
Available Files |
Plaque Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |