| Detailed Information for Phage Loser |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | L0S3R |
| Found By | Lissenya B. Argueta |
| Year Found | 2009 |
| Location Found | Provo Canyon, UT USA |
| Finding Institution | Brigham Young University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.32945 N, 111.621583 W Map |
| Discovery Notes | Found on 1 Sep. 2009 around 2:00 p.m. on a riverbank about two inches into the soil. The approx. temp. was 65°F. Soil was loose and very moist. Tree roots and dead bugs were also around the sample. |
| Naming Notes | Frustration with the isolation process. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 18, 2012 |
| Sequencing Facility | BYU DNA Sequencing Center |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 224 |
| Genome length (bp) | 53486 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTAA |
| GC Content | 64.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Small and turbid, approx. 1mm in diameter after 48 hours. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU761558 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2009BRYO10s3rLA |
| SEA Lysate Titer |
2e8 |
| Date of SEA Lysate Titering |
Dec 22, 2010 |
| Pitt Freezer Box# |
11 |
| Pitt Freezer Box Grid# |
67 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
