| Detailed Information for Phage Spino |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ethan West |
| Year Found | 2022 |
| Location Found | Holland, MI United States |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 0.427875 N, 0.861089 W Map |
| Discovery Notes | Isolated from a sample of soil found about two inches under the topsoil at the roots of a walnut tree in the pine grove of Hope college. |
| Naming Notes | Named after the Spinosaurus, the apex predator of land and sea! Well, they technically ate small fish and not much else but they are still very fascinating creatures that deserve more recognition. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 13, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 728 |
| Genome length (bp) | 51372 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Phage Spino does not seem to exhibit any form of temperate behavior. Based on several tests for a lysogen resulting in none forming as well as the plaque morphology always being clear creates a moderate level of confidence in this claim. |
| Other Cluster Members |
|
| Annotating Institution | Hope College |
| Annotation Status | Being Annotated (Expected completion by 5/1/2023) |
| Plaque Notes | The plaques are about 1mm in diameter with a small variation in size throughout an average plate. The edges taper off to a small turbid ring and no external morphology such as rings or tails have ever been observed. |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
151 |
| Pitt Freezer Box Grid# |
H1 |
| Available Files |
| Restriction Digest Picture | Download |