| Detailed Information for Phage Kipper29 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Mary Marino |
| Year Found | 2018 |
| Location Found | Philadelphia, PA United States of America |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 29°C |
| GPS Coordinates | 39.995556 N, 75.236944 W Map |
| Discovery Notes | The sample was collected on 9/2/18 at 2:27pm under a tree east of Mandeville Hall at Saint Joseph's University. The soil was dry but cool, and was found 3.5-4.0 cm underground. The ambient temperature was 29ºC with 67% humidity; it was sunny, and had rained two days before. |
| Naming Notes | I named my phage after a character from a television show I used to watch when I was little, "Kipper the Dog." The theme song was stuck in my head the day we were asked to name the phage. The "29" is a reference to my sister's birthday. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 26, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2054 |
| Genome length (bp) | 52009 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotation Status | Awaiting_QC |
| Plaque Notes | The plaques were mostly turbid and slightly cloudy, indicating a temperate phage. The average size of the plaques was between 3 mm and 5 mm in diameter after 24-48 hours in the incubator at 37ºC. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
85 |
| Pitt Freezer Box Grid# |
E1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
