| Detailed Information for Phage Bartimeaus |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Marcus Barnett |
| Year Found | 2016 |
| Location Found | Ferguson, KY USA |
| Finding Institution | Somerset Community College |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.05658 N, 84.616182 W Map |
| Discovery Notes | My second soil sample was collected from the back yard of my dad’s house in Ferguson. It was in a nice open area that gets plenty of sun and air, the weather was a nice 65̊ F and the soil was slightly damp. This sample worked on the first try. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 27, 2017 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 290 |
| Genome length (bp) | 51374 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Somerset Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Size (mm): 3mm diameter
Appearance (clear, turbid etc..): Thin-turbid, almost clear |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT889390 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.0x10^8 pfu/mL |
| Date of SEA Lysate Titering |
Apr 21, 2016 |
| Pitt Freezer Box# |
28 |
| Pitt Freezer Box Grid# |
A12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
