Detailed Information for Phage Vix |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Megan Ludwig |
Year Found | 2009 |
Location Found | Holland, MI USA |
Finding Institution | Hope College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 42.789061 N, 86.100782 W Map |
Discovery Notes | 3 inches from drain; on small incline facing east. Soil was dark, with a little moisture and sandy in quality. Vegetation was present in the soil. 2 ft away from a sidewalk and 7 ft away from Dykstra Hall. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Mar 17, 2011 |
Sequencing Facility | Joint Genomics Institute |
Genome length (bp) | 50963 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGGTGGTAA |
GC Content | 64.0% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A3 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Plaques are typically 3-5mm in diameter but also a few are 1-2mm. They are turbid with a comet-tailed ring. This morphology is apparent after 24hrs in incubator at 37°C with little change after 48 hrs at room temperature. |
Morphotype | Siphoviridae |
Number of Genes | 82 |
Number of tRNAs | 3 |
Has been Phamerated? | Yes |
Gene List |
|
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | JF704114 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
2009HOPEvixMLL |
SEA Lysate Titer |
1.4e10 |
Pitt Freezer Box# |
3 |
Pitt Freezer Box Grid# |
C10 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
Virtual Digest Picture | Download |
EM Picture | Download |