| Detailed Information for Phage Vix |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Megan Ludwig |
| Year Found | 2009 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.789061 N, 86.100782 W Map |
| Discovery Notes | 3 inches from drain; on small incline facing east. Soil was dark, with a little moisture and sandy in quality. Vegetation was present in the soil. 2 ft away from a sidewalk and 7 ft away from Dykstra Hall. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 17, 2011 |
| Sequencing Facility | Joint Genomics Institute |
| Genome length (bp) | 50963 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGTGGTAA |
| GC Content | 64.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are typically 3-5mm in diameter but also a few are 1-2mm. They are turbid with a comet-tailed ring. This morphology is apparent after 24hrs in incubator at 37°C with little change after 48 hrs at room temperature. |
| Morphotype | Siphoviridae |
| Number of Genes | 82 |
| Number of tRNAs | 3 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | JF704114 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2009HOPEvixMLL |
| SEA Lysate Titer |
1.4e10 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
C10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
