| Detailed Information for Phage LittleB |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sucely Ponce and Chikaodi Obioha |
| Year Found | 2015 |
| Location Found | Nyack, NY USA |
| Finding Institution | Nyack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 41.084175 N, 73.928556 W Map |
| Discovery Notes | Soil type was brown-colored, rocky dirt. It was collected in an area surrounded by rocks and shrubs, where people step on it daily.
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 15, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1048 |
| Genome length (bp) | 51373 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotation Status | In GenBank |
| Plaque Notes | Plaque Morphology (after 24hrs):
Diameter: 2mm
Clarity of Plaque: Clear
|
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX817174 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.4 x 10^9 pfu/mL |
| Date of SEA Lysate Titering |
Oct 26, 2015 |
| Pitt Freezer Box# |
22 |
| Pitt Freezer Box Grid# |
G8 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |