Detailed Information for Phage LittleB |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Sucely Ponce and Chikaodi Obioha |
Year Found | 2015 |
Location Found | Nyack, NY USA |
Finding Institution | Nyack College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 41.084175 N, 73.928556 W Map |
Discovery Notes | Soil type was brown-colored, rocky dirt. It was collected in an area surrounded by rocks and shrubs, where people step on it daily.
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Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Dec 15, 2015 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1048 |
Genome length (bp) | 51373 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGCCGGTAA |
GC Content | 63.9% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A4 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Plaque Morphology (after 24hrs): Diameter: 2mm Clarity of Plaque: Clear
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Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
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Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KX817174 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
3.4 x 10^9 pfu/mL |
Date of SEA Lysate Titering |
Oct 26, 2015 |
Pitt Freezer Box# |
22 |
Pitt Freezer Box Grid# |
G8 |
Available Files |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |