| Detailed Information for Phage Ruin |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Xavier Brickeen |
| Year Found | 2015 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.985639 N, 86.455444 W Map |
| Discovery Notes | The soil was gathered from dry soil underneath a tree was approximately 4 centimeters deep. The tree was located 1 meter away from a concrete sidewalk and 4 meters away from a building. |
| Naming Notes | I came up with the name Ruin because when a phage is in the lytic cycle and breaks out of the host cell, it "ruins" the cell. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 1, 2016 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 287 |
| Genome length (bp) | 51365 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | The plaques are slightly turbid. |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | When incubated at 37 degrees Celsius, plaques are slightly turbid and approximately 2.5 millimeters in diameter. The plaques can be seen after 24 hours incubation and do not change after 48 hours. When incubated at 30 degrees Celsius the plaque morphology does not change other then becoming slightly more turbid. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH825710 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.25x10^11 |
| Pitt Freezer Box# |
27 |
| Pitt Freezer Box Grid# |
F7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
