Mycobacterium phage Kanely
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Detailed Information for Phage Kanely
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKhayla Easely
Year Found2016
Location FoundChamplain, VA USA
Finding InstitutionVirginia Union University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates38.010007 N, 77.004354 W Map
Discovery NotesThis sample was extracted near a boating dock close to the Potomac River. This was a soil sample, from flowerbed, with a depth of ~4.5 inches. The ambient temperature was 80 degrees (F), sunny on September 3, 2016.
Naming NotesKanely was derived from a 3 person group (Khayla, Natasha and Lizzy).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3518
Genome length (bp)52539
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGTAA
GC Content63.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA1
Cluster Life CycleTemperate
Other Cluster Members

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Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Ajay (A1)

Alsfro (A1)

Alvin (A1)

Anglerfish (A1)

Arcanine (A1)

Arlo (A1)

Atkinbua (A1)

BaconJack (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Bexan (A1)

Big3 (A1)

Bigfoot (A1)

BigMau (A1)

BigPaolini (A1)

BillKnuckles (A1)

Bircsak (A1)

Blue (A1)

Bob3 (A1)

Bones (A1)

BPBiebs31 (A1)

Briton15 (A1)

Bruns (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Carlyle (A1)

ConceptII (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

DrFeelGood (A1)

Dulcie (A1)

Dynamix (A1)

Edtherson (A1)

Euphoria (A1)

Fajezeel (A1)

Fascinus (A1)

Fenn (A1)

Forsytheast (A1)

Francis47 (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gompeii16 (A1)

Graduation (A1)

Greg (A1)

HanShotFirst (A1)

HarryOW (A1)

HermioneGrange (A1)

Homines (A1)

Hope4ever (A1)

Ichabod (A1)

ILeeKay (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

Jerm2 (A1)

Kanely (A1)

KBG (A1)

Killigrew (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Lockley (A1)

Lopton (A1)

Magnar (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Maroc7 (A1)

MaryBeth (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Moose (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

Naira (A1)

NEHalo (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Oogway (A1)

PacerPaul (A1)

Papez (A1)

Paphu (A1)

Pari (A1)

PattyP (A1)

Pepe (A1)

Perseus (A1)

Petp2012 (A1)

Petruchio (A1)

PherrisBueller (A1)

PhrostyMug (A1)

PinkPlastic (A1)

Pinto (A1)

Pippin (A1)

Pita2 (A1)

PSullivan (A1)

Rajelicia (A1)

Rhynn (A1)

RidgeCB (A1)

Ringer (A1)

Rohr (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

SarFire (A1)

Scowl (A1)

Seabiscuit (A1)

Sibs6 (A1)

SkiPole (A1)

Smairt (A1)

Smeagol (A1)

Snazzy (A1)

Solon (A1)

SpikeBT (A1)

Squee (A1)

StrongArm (A1)

Sumter (A1)

SwissCheese (A1)

Switzer (A1)

Target (A1)

Tasp14 (A1)

TheloniousMonk (A1)

Thor (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

U2 (A1)

Violet (A1)

Watermelon (A1)

Wheeler (A1)

Wilkins (A1)

Zeeculate (A1)

Zephyr (A1)

Zeuska (A1)

Chupacabra (A10)

Drake94 (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

OKCentral2016 (A10)

Rebeuca (A10)

RhynO (A10)

Severus (A10)

Topanga (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Aneem (A11)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Hutc2 (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Salz (A11)

Snape (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Phlei (A13)

Jeeves (A14)

Luchador (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

MinecraftSteve (A15)

Nedarya (A15)

ReMo (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Waits (A15)

EagleEye (A16)

Lucyedi (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

AN3 (A2)

AN9 (A2)

ANI8 (A2)

AnnaL29 (A2)

Anselm (A2)

ArcherNM (A2)

Bactobuster (A2)

Baehexic (A2)

Benvolio (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Bugsy (A2)

Centaur (A2)

Changeling (A2)

Che12 (A2)

ChipMunk (A2)

CRB1 (A2)

Crucio (A2)

D29 (A2)

D32 (A2)

Dalmatian (A2)

DBQu4n (A2)

Drake55 (A2)

DudeLittle (A2)

Echild (A2)

Equemioh13 (A2)

EvilGenius (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

Heffalump (A2)

Herbertwm (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kalpine (A2)

Kerberos (A2)

L5 (A2)

LadyBird (A2)

Larenn (A2)

Leogania (A2)

LilTurb (A2)

Lokk (A2)

Loser (A2)

Malec (A2)

Miko (A2)

MissWhite (A2)

Naji (A2)

NaSiaTalie (A2)

NicoleTera (A2)

NothingSpecial (A2)

Odin (A2)

Ph8s (A2)

Phaded (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Rachaly (A2)

RedRock (A2)

Retro23 (A2)

SemperFi (A2)

Serenity (A2)

SnapTap (A2)

StarStuff (A2)

Superchunk (A2)

SweetiePie (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

Turbido (A2)

Updawg (A2)

VA6 (A2)

VC3 (A2)

Whabigail7 (A2)

WideWale (A2)

XianYue (A2)

Anthony (A20)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

AugsMagnumOpus (A3)

BabyRay (A3)

Beauxregard13 (A3)

BreSam8 (A3)

BuzzBuzz (A3)

Bxz2 (A3)

Caviar (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Groupthink (A3)

Grub (A3)

Grum1 (A3)

GtownJaz (A3)

Heathen (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

Isca (A3)

JenCasNa (A3)

JeppNRM (A3)

JF2 (A3)

JF4 (A3)

JHC117 (A3)

Jobu08 (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

Lambert1 (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

MA5 (A3)

MadMarie (A3)

Mainiac (A3)

Malinsilva (A3)

Margo (A3)

Marie (A3)

Marius (A3)

MarQuardt (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MK4 (A3)

MoneyMay (A3)

MuchMore (A3)

Noella (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

P28Green (A3)

Panamaxus (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

Rockstar (A3)

Sabia (A3)

Sabinator (A3)

SoilDragon (A3)

Soshari (A3)

Spike509 (A3)

Stagni (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

Backyardigan (A4)

Badger (A4)

Bartimeaus (A4)

BellusTerra (A4)

Blackmoor (A4)

Bombshell (A4)

Broseidon (A4)

Bruiser (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Cici (A4)

Cindaradix (A4)

Cintron (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

Datway (A4)

Deano (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Florean (A4)

Flux (A4)

Funston (A4)

Gadost (A4)

HamSlice (A4)

Holli (A4)

Houdini22 (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Kratark (A4)

Kyee (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

LittleB (A4)

LittleGuy (A4)

Lorenzo (A4)

Maverick (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Miramae (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nebs (A4)

Nemo27 (A4)

Noelle (A4)

NorthStar (A4)

NotAPhaseMom (A4)

Nyxis (A4)

Obama12 (A4)

Ohfah (A4)

OKaNui (A4)

Palestino (A4)

Peaches (A4)

PeterPeter (A4)

Phacado (A4)

Phighter1804 (A4)

Phontbonne (A4)

Pipcraft (A4)

Polymorphads (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Ruin (A4)

Sabertooth (A4)

Scamp (A4)

Shaka (A4)

SheldonCooper (A4)

Skipitt (A4)

Stasia (A4)

Thanksgivukkah (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

TroyPia (A4)

TygerBlood (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Benedict (A5)

Bluefalcon (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Dublin (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Midas2 (A5)

Naca (A5)

PetterN (A5)

Phlorence (A5)

Scorpia (A5)

Swirley (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

Zolita (A5)

Artemis2UCLA (A6)

Blinn1 (A6)

Blue7 (A6)

CloudWang3 (A6)

DaVinci (A6)

EricB (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Hexamo (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Kipper29 (A6)

Koko (A6)

McFly (A6)

Pmask (A6)

Priamo (A6)

SmellyB (A6)

SuperAwesome (A6)

ToneTone (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

DroogsArmy (A7)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Danforth (A8)

Dixon (A8)

Expelliarmus (A8)

NearlyHeadless (A8)

Roary (A8)

Saintus (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Arissanae (A9)

Beemo (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

DreamTeam1 (A9)

Eidsmoe (A9)

Elephantoon (A9)

ExplosioNervosa (A9)

HortumSL17 (A9)

Keziacharles14 (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

SheaKeira (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesThe average plaque size is ~0.425 cm. There is a halo/turbidity effect, suggesting a lysogenic phenotype.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Kanely_1

Kanely_2

Kanely_3
(HNH endonuclease)

Kanely_4
(terminase, small subunit)

Kanely_5

Kanely_6
(minor tail protein)

Kanely_7

Kanely_8
(minor tail protein)

Kanely_9

Kanely_10
(lysin A)

Kanely_11
(lysin B)

Kanely_12
(terminase, large subunit)

Kanely_13
(portal protein)

Kanely_14
(caspid maturation protease)

Kanely_15
(scaffolding protein)

Kanely_16
(major capsid protein)

Kanely_17

Kanely_18
(head-to-tail adaptor)

Kanely_19
(head-to-tail stopper)

Kanely_20

Kanely_21
(tail terminator)

Kanely_22
(minor tail protein)

Kanely_23
(tail assembly chaperone)

Kanely_24
(tail asembly chaperone)

Kanely_25
(tape measure protein)

Kanely_26
(minor tail protein)

Kanely_27

Kanely_28
(minor tail protein)

Kanely_29

Kanely_30

Kanely_31

Kanely_32
(minor tail protein)

Kanely_33
(minor tail protein)

Kanely_34

Kanely_35

Kanely_36

Kanely_37

Kanely_38
(serine integrase)

Kanely_39

Kanely_40

Kanely_41

Kanely_42

Kanely_43

Kanely_44

Kanely_45
(DNA polymerase I)

Kanely_46

Kanely_47

Kanely_48

Kanely_49

Kanely_50

Kanely_51
(RNA polymerase sigma factor)

Kanely_52

Kanely_53
(Recombination directionality factor)

Kanely_54

Kanely_55

Kanely_56

Kanely_57
(DNA primase)

Kanely_58
(DNA primase)

Kanely_59
(DNA primase)

Kanely_60

Kanely_61
(endonuclease VII)

Kanely_62

Kanely_63
(NrdH-like glutaredoxin)

Kanely_64
(DnaB-like dsDNA helicase)

Kanely_65

Kanely_66

Kanely_67
(helix-turn-helix DNA binding domain)

Kanely_68

Kanely_69
(helix-turn-helix DNA binding domain, MerR-like)

Kanely_70
(cas4 family exonuclease)

Kanely_71

Kanely_72

Kanely_73

Kanely_74

Kanely_75
(membrane protein)

Kanely_76

Kanely_77
(immunity repressor)

Kanely_78

Kanely_79
(membrane protein)

Kanely_80

Kanely_81

Kanely_82

Kanely_83

Kanely_84

Kanely_85
(membrane protein)

Kanely_86

Kanely_87

Kanely_88

Kanely_89

Kanely_90

Kanely_91
(DNA methylase)

Kanely_92

Kanely_93
(membrane protein)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN585976
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.125 x 10^10
Date of SEA Lysate Titering Nov 29, 2016
Pitt Freezer Box# 33
Pitt Freezer Box Grid# G6
Available Files
Plaque PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload