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Mycobacterium phage Big3
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Detailed Information for Phage Big3
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBrandon Brown-Ruffin
Year Found2016
Location FoundRichmond, VA USA
Finding InstitutionVirginia Union University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.6 N, 77.42 W Map
Discovery NotesSample was isolated from damp soil.
Naming NotesA 3 person group processed this phage sample, and are avid Laker fans. The Lakers have at least 3 superstar athletes to include Shaq, Kobe and Magic Johnson. Therefore, the name is an ode to the 3 most famous Lakers basketball players.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1741
Genome length (bp)53442
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGTAA
GC Content63.4%
Sequencing NotesThere are a couple of SNPs in the reads. At position 16420, about 48% of the reads have a G instead of the called A. At position 16433, about 48% of the reads have a G instead of the called A.

There is also, in about 40% of the reads, a deletion that is 1068 bp long from position 43969 to position 45036, inclusive.
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA1
Cluster Life CycleTemperate
Other Cluster Members

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Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Ajay (A1)

Alsfro (A1)

Alvin (A1)

Anglerfish (A1)

Arcanine (A1)

Arlo (A1)

Atkinbua (A1)

BaconJack (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Bexan (A1)

Big3 (A1)

Bigfoot (A1)

BigMau (A1)

BigPaolini (A1)

BillKnuckles (A1)

Bircsak (A1)

Blue (A1)

Bob3 (A1)

Bones (A1)

BPBiebs31 (A1)

Briton15 (A1)

Bruns (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Carlyle (A1)

ConceptII (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

DrFeelGood (A1)

Dulcie (A1)

Dynamix (A1)

Edtherson (A1)

Euphoria (A1)

Fajezeel (A1)

Fascinus (A1)

Fenn (A1)

Forsytheast (A1)

Francis47 (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gompeii16 (A1)

Graduation (A1)

Greg (A1)

HanShotFirst (A1)

HarryOW (A1)

HermioneGrange (A1)

Homines (A1)

Hope4ever (A1)

Ichabod (A1)

ILeeKay (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

Jerm2 (A1)

Kanely (A1)

KBG (A1)

Killigrew (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Lockley (A1)

Lopton (A1)

Magnar (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Maroc7 (A1)

MaryBeth (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Moose (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

Naira (A1)

NEHalo (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Oogway (A1)

PacerPaul (A1)

Papez (A1)

Paphu (A1)

Pari (A1)

PattyP (A1)

Pepe (A1)

Perseus (A1)

Petp2012 (A1)

Petruchio (A1)

PherrisBueller (A1)

PhrostyMug (A1)

PinkPlastic (A1)

Pinto (A1)

Pippin (A1)

Pita2 (A1)

PSullivan (A1)

Rajelicia (A1)

Rhynn (A1)

RidgeCB (A1)

Ringer (A1)

Rohr (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

SarFire (A1)

Scowl (A1)

Seabiscuit (A1)

Sibs6 (A1)

SkiPole (A1)

Smairt (A1)

Smeagol (A1)

Snazzy (A1)

Solon (A1)

SpikeBT (A1)

Squee (A1)

StrongArm (A1)

Sumter (A1)

SwissCheese (A1)

Switzer (A1)

Target (A1)

Tasp14 (A1)

TheloniousMonk (A1)

Thor (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

U2 (A1)

Violet (A1)

Watermelon (A1)

Wheeler (A1)

Wilkins (A1)

Zeeculate (A1)

Zephyr (A1)

Zeuska (A1)

Chupacabra (A10)

Drake94 (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

OKCentral2016 (A10)

Rebeuca (A10)

RhynO (A10)

Severus (A10)

Topanga (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Aneem (A11)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Hutc2 (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Salz (A11)

Snape (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Phlei (A13)

Jeeves (A14)

Luchador (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

MinecraftSteve (A15)

Nedarya (A15)

ReMo (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Waits (A15)

EagleEye (A16)

Lucyedi (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

AN3 (A2)

AN9 (A2)

ANI8 (A2)

AnnaL29 (A2)

Anselm (A2)

ArcherNM (A2)

Bactobuster (A2)

Baehexic (A2)

Benvolio (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Bugsy (A2)

Centaur (A2)

Changeling (A2)

Che12 (A2)

ChipMunk (A2)

CRB1 (A2)

Crucio (A2)

D29 (A2)

D32 (A2)

Dalmatian (A2)

DBQu4n (A2)

Drake55 (A2)

DudeLittle (A2)

Echild (A2)

Equemioh13 (A2)

EvilGenius (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

Heffalump (A2)

Herbertwm (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kalpine (A2)

Kerberos (A2)

L5 (A2)

LadyBird (A2)

Larenn (A2)

Leogania (A2)

LilTurb (A2)

Lokk (A2)

Loser (A2)

Malec (A2)

Miko (A2)

MissWhite (A2)

Naji (A2)

NaSiaTalie (A2)

NicoleTera (A2)

NothingSpecial (A2)

Odin (A2)

Ph8s (A2)

Phaded (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Rachaly (A2)

RedRock (A2)

Retro23 (A2)

SemperFi (A2)

Serenity (A2)

SnapTap (A2)

StarStuff (A2)

Superchunk (A2)

SweetiePie (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

Turbido (A2)

Updawg (A2)

VA6 (A2)

VC3 (A2)

Whabigail7 (A2)

WideWale (A2)

XianYue (A2)

Anthony (A20)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

AugsMagnumOpus (A3)

BabyRay (A3)

Beauxregard13 (A3)

BreSam8 (A3)

BuzzBuzz (A3)

Bxz2 (A3)

Caviar (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Groupthink (A3)

Grub (A3)

Grum1 (A3)

GtownJaz (A3)

Heathen (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

Isca (A3)

JenCasNa (A3)

JeppNRM (A3)

JF2 (A3)

JF4 (A3)

JHC117 (A3)

Jobu08 (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

Lambert1 (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

MA5 (A3)

MadMarie (A3)

Mainiac (A3)

Malinsilva (A3)

Margo (A3)

Marie (A3)

Marius (A3)

MarQuardt (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MK4 (A3)

MoneyMay (A3)

MuchMore (A3)

Noella (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

P28Green (A3)

Panamaxus (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

Rockstar (A3)

Sabia (A3)

Sabinator (A3)

SoilDragon (A3)

Soshari (A3)

Spike509 (A3)

Stagni (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

Backyardigan (A4)

Badger (A4)

Bartimeaus (A4)

BellusTerra (A4)

Blackmoor (A4)

Bombshell (A4)

Broseidon (A4)

Bruiser (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Cici (A4)

Cindaradix (A4)

Cintron (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

Datway (A4)

Deano (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Florean (A4)

Flux (A4)

Funston (A4)

Gadost (A4)

HamSlice (A4)

Holli (A4)

Houdini22 (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Kratark (A4)

Kyee (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

LittleB (A4)

LittleGuy (A4)

Lorenzo (A4)

Maverick (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Miramae (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nebs (A4)

Nemo27 (A4)

Noelle (A4)

NorthStar (A4)

NotAPhaseMom (A4)

Nyxis (A4)

Obama12 (A4)

Ohfah (A4)

Palestino (A4)

Peaches (A4)

PeterPeter (A4)

Phacado (A4)

Phighter1804 (A4)

Phontbonne (A4)

Pipcraft (A4)

Polymorphads (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Ruin (A4)

Sabertooth (A4)

Scamp (A4)

Shaka (A4)

SheldonCooper (A4)

Skipitt (A4)

Stasia (A4)

Thanksgivukkah (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

TroyPia (A4)

TygerBlood (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Benedict (A5)

Bluefalcon (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Dublin (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Midas2 (A5)

Naca (A5)

PetterN (A5)

Phlorence (A5)

Scorpia (A5)

Swirley (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

Zolita (A5)

Artemis2UCLA (A6)

Blinn1 (A6)

Blue7 (A6)

CloudWang3 (A6)

DaVinci (A6)

EricB (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Hexamo (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Kipper29 (A6)

Koko (A6)

McFly (A6)

Pmask (A6)

Priamo (A6)

SuperAwesome (A6)

ToneTone (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Danforth (A8)

Dixon (A8)

Expelliarmus (A8)

NearlyHeadless (A8)

Roary (A8)

Saintus (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Arissanae (A9)

Beemo (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

DreamTeam1 (A9)

Eidsmoe (A9)

Elephantoon (A9)

ExplosioNervosa (A9)

HortumSL17 (A9)

Keziacharles14 (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

SheaKeira (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotating InstitutionUniversity of Lagos
Annotation StatusIn GenBank
Plaque NotesThe average plaque size is ~0.6 cm. There are some slightly smaller plaques ~0.2 cm diameter that consistently appear throughout the purification, suggesting a mixed phenotype. However, each is consistent in producing a turbid plaque.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Big3_1
(HNH endonuclease)

Big3_2

Big3_3
(terminase small subunit)

Big3_4

Big3_5
(minor tail protein)

Big3_6

Big3_7
(minor tail protein)

Big3_8

Big3_9
(lysin A)

Big3_10
(lysin B)

Big3_11
(terminase large subunit)

Big3_12
(portal protein)

Big3_13
(capsid maturation protease)

Big3_14
(scaffolding protein)

Big3_15
(major capsid protein)

Big3_16

Big3_17
(head-to-tail adaptor)

Big3_18
(head-to-tail stopper)

Big3_19

Big3_20
(tail terminator)

Big3_21
(major tail protein)

Big3_22
(tail assembly chaperone)

Big3_23
(tail assembly chaperone)

Big3_24
(tape measure protein)

Big3_25
(minor tail protein)

Big3_26

Big3_27
(minor tail protein)

Big3_28

Big3_29

Big3_30

Big3_31
(minor tail protein)

Big3_32
(minor tail protein)

Big3_33

Big3_34

Big3_35

Big3_36

Big3_37
(serine integrase)

Big3_38

Big3_39

Big3_40

Big3_41

Big3_42

Big3_43
(DNA polymerase I)

Big3_44

Big3_45

Big3_46

Big3_47

Big3_48

Big3_49
(RNA polymerase sigma factor)

Big3_50

Big3_51
(recombination directionality factor)

Big3_52

Big3_53

Big3_54
(DNA primase)

Big3_55
(DNA primase)

Big3_56

Big3_57

Big3_58
(endonuclease VII)

Big3_59

Big3_60

Big3_61
(HNH endonuclease)

Big3_62

Big3_63
(NrdH-like glutaredoxin)

Big3_64
(DnaB-like dsDNA helicase)

Big3_65

Big3_66
(helix-turn-helix DNA binding domain protein)

Big3_67
(MerR-like helix-turn-helix DNA binding domain protein)

Big3_68
(methyltransferase)

Big3_69

Big3_70
(cas4 family exonuclease)

Big3_71

Big3_72

Big3_73

Big3_74
(membrane protein)

Big3_75

Big3_76
(immunity repressor)

Big3_77

Big3_78

Big3_79

Big3_80

Big3_81

Big3_82
(membrane protein)

Big3_83

Big3_84

Big3_85

Big3_86

Big3_87
(DNA methylase)

Big3_88

Big3_89

Big3_90

Big3_91
(membrane protein)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK967387
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.3 x 10^10
Date of SEA Lysate Titering Nov 29, 2016
Pitt Freezer Box# 33
Pitt Freezer Box Grid# G5
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload