Detailed Information for Phage BuzzBuzz |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Hannah Chatwin and Annie Waddle |
Year Found | 2015 |
Location Found | Boulder, CO USA |
Finding Institution | University of Colorado at Boulder |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 40.007600 N, 105.265900 W Map |
Discovery Notes | Chi Psi Fraternity House- muddy, watery puddle surrounded by wasps |
Naming Notes | We chose this name because we found our phage in a puddle surrounded by wasps. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 15, 2016 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 300 |
Genome length (bp) | 50897 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGGTGGTAA |
GC Content | 64.2% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A3 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Small, lytic, ≤1mm |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KX523125 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
3.7 x 1010 pfu/mL |
Date of SEA Lysate Titering |
Oct 19, 2015 |
Pitt Freezer Box# |
24 |
Pitt Freezer Box Grid# |
H5 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |