| Detailed Information for Phage Tinybot |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Phaggybot |
| Found By | Chenyu Zhang |
| Year Found | 2015 |
| Location Found | Easton, PA USA |
| Finding Institution | Lafayette College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.698889 N, 75.206944 W Map |
| Discovery Notes | Damp soil with sprinkler on nearby; 2cm under topsoil |
| Naming Notes | A bot is a simple computer program used to perform highly repetitive operations. I like to think of my phage Tinybot as a tiny biological machine. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2929 |
| Genome length (bp) | 51402 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 64.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, round plaques with 2mm diameter |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KY549155 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.8 x 10^9 pfu/ml |
| Date of SEA Lysate Titering |
Oct 20, 2015 |
| Pitt Freezer Box# |
20 |
| Pitt Freezer Box Grid# |
A9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
