Detailed Information for Phage Tinybot |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Former names | Phaggybot |
Found By | Chenyu Zhang |
Year Found | 2015 |
Location Found | Easton, PA USA |
Finding Institution | Lafayette College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 40.698889 N, 75.206944 W Map |
Discovery Notes | Damp soil with sprinkler on nearby; 2cm under topsoil |
Naming Notes | A bot is a simple computer program used to perform highly repetitive operations. I like to think of my phage Tinybot as a tiny biological machine. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Dec 16, 2015 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 2929 |
Genome length (bp) | 51402 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGCCGGTAA |
GC Content | 64.0% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A4 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Clear, round plaques with 2mm diameter |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KY549155 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
3.8 x 10^9 pfu/ml |
Date of SEA Lysate Titering |
Oct 20, 2015 |
Pitt Freezer Box# |
20 |
Pitt Freezer Box Grid# |
A9 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |