Detailed Information for Phage Zeuska |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Emma Herold |
Year Found | 2013 |
Location Found | Providence, RI USA |
Finding Institution | Brown University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 41.824047 N, 71.403114 W Map |
Discovery Notes | Sample obtained from cool soil lying under a tanbark top layer, all found underneath an overhanging tree. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Dec 18, 2013 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 3844 |
Genome length (bp) | 53598 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGATGGTAA |
GC Content | 63.7% |
Sequencing Notes | Some of the reads from this project indicate that a portion of the genome has been deleted in a subset of the population. About 15% of the reads show this deletion. The deletion is 1803 bp and runs from position 26961 to 28763 (inclusive). There is a 12 bp sequence (CGGGCTGCTGGG) located near these two positions that is collapsed into one copy in the deletion version of the genome. |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | A |
Subcluster | A1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | Tarleton State University |
Annotation Status | In GenBank |
Plaque Notes | Predicted cluster type: D, F1, or F2 |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
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Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK524506 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
1.1x10^10 |
Pitt Freezer Box# |
4 |
Pitt Freezer Box Grid# |
E11 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |