| Detailed Information for Phage Adahisdi |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Aaron Stevens |
| Year Found | 2015 |
| Location Found | Cullowhee, NC USA |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.32 N, 83.17 W Map |
| Discovery Notes | Collected from a compost pile. |
| Naming Notes | This phage was presented to the class as a murderer of bacteria. The native american Cherokee tribe once inhabited the region this phage was found. Adahisdi is the Cherokee word for murder. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 9, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3157 |
| Genome length (bp) | 51703 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Carolina University |
| Annotation Status | In GenBank |
| Plaque Notes | This phage makes two different plaques, both lytic in appearance. About half of the plaques are medium/large, and half are small. |
| Morphotype | Siphoviridae |
| Number of Genes | 84 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH697575 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.0x10^9 |
| Date of SEA Lysate Titering |
Oct 28, 2015 |
| Pitt Freezer Box# |
19 |
| Pitt Freezer Box Grid# |
F10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
