Mycobacterium phage AvatarAhPeg
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Detailed Information for Phage AvatarAhPeg
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByZachary Woessner
Year Found2014
Location FoundProvidence, RI USA
Finding InstitutionBrown University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.825683 N, 71.403061 W Map
Discovery NotesFound on the Brown University main green under the fourth tree on the left coming from the gate that opens onto Brown Street (southern end).
Naming NotesThe presentation was a parody of Avatar: The Last Airbender, a TV series. The phage was named AvatarAhPeg--AhPeg being an anagram for phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 17, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage2753
Genome length (bp)51637
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGGTAA
GC Content63.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA4
Cluster Life CycleTemperate
Lysogeny NotesThe plaques are temperate (being turbid). The plaques often have a clear, lytic center.
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesDepending upon the condition of the M. smeg, the plaques changed. In yellow smeg, the plaques were ~.55mm in diameter and completely temperate. In normal, clear smeg, the plaques were temperate with a lytic center (bullseyes) and ~.3mm in diameter.
MorphotypeSiphoviridae
Number of Genes89
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK494129
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.5x10^10
Date of SEA Lysate Titering Nov 7, 2014
Pitt Freezer Box# 11
Pitt Freezer Box Grid# E8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload