| Detailed Information for Phage Burger |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Mitchell Richards, Logan Bond |
| Year Found | 2013 |
| Location Found | Arkadelphia, AR USA |
| Finding Institution | Ouachita Baptist University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.127526 N, 93.050262 W Map |
| Discovery Notes | Found 8/26/13 @12:15p.m.
87 deg F
7.62 cm depth
Fairly dry soil
Mostly in shade
Near water, wooded area, approx. 30ft. from path
Slight slope facing east |
| Naming Notes | From our love for burgers. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 27, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 95 |
| Sanger Finishing Reactions | 12 |
| Genome length (bp) | 51371 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | 1-3 mm in diameter
clear, crisp centers with cloudy edges
small "comet" effect - where cloudy edge trails in one direction on random plaques (no real order to why)
|
| Morphotype | Siphoviridae |
| Number of Genes | 86 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU985094 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.6x10^9 pfu/mL |
| Date of SEA Lysate Titering |
Oct 10, 2013 |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
E1 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
