| Detailed Information for Phage Changeling |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Delaney O'Malley |
| Year Found | 2013 |
| Location Found | Pullman, WA USA |
| Finding Institution | Washington State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 46.73003 N, 117.16543 W Map |
| Discovery Notes | The soil came from the surface of a dry patch of dirt under a tree on 09/10/13. |
| Naming Notes | A changeling is a mythical creature that is the deformed child of a fairy which is put into a mortal's household in exchange for their own child. The changeling then causes havoc inside the mortal's household. This reminded me of the way that some phage cause bacteria to become pathogenic once their DNA is incorporated into the bacterial DNA. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 18, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 133 |
| Sanger Finishing Reactions | 12 |
| Genome length (bp) | 52991 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 64.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques range in size from about 2 mm to 5 mm diameters. They are circular and show both a bulls eye pattern and comet tail characteristic. This causes the plaques to be both clear and turbid. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140403 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.69 x 10^9 pfu/mL |
| Date of SEA Lysate Titering |
Nov 5, 2013 |
| Pitt Freezer Box# |
4 |
| Pitt Freezer Box Grid# |
A5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
