Mycobacterium phage Chaph
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Detailed Information for Phage Chaph
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesChaphph (rejected)
Found ByJonathan Mathews, Collin Patterson, Benjamin Tebbe
Year Found2019
Location FoundLongview, TX USA
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature26°C
GPS Coordinates32.466 N, 94.731 W Map
Discovery NotesHumidity was 90% at the time of sample collection. The sample was evidently sandy, but there were signs of mulch use. The soil was collected approximately 8 inches away from the base of a tree.
Naming Notes'Chaph' comes from chaff, a Biblical reference to the undesirable side-product of wheat. Historically, chaff serves no purpose. Initially, we were told the soil was undesirable, like chaff. Ironically, this soil was the only sample of five to be successful.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina MiSeq
Read TypeSingle-end reads
Read Length150 bp
Approximate Shotgun Coverage1055
Genome length (bp)51383
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGGTAA
GC Content63.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA4
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLeTourneau University
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes89
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT498056
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 101
Pitt Freezer Box Grid# E12
Available Files
GenBank File for PhameratorDownload