Mycobacterium phage DaHudson
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Skelbel (AZ1) Keough (EK2) MamaT (GI) Lilo27 (EB) Liberone (GI)Mycobacterium phage DaHudson
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| Detailed Information for Phage DaHudson | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | TheHudson |
| Found By | Christine Kline and Sarah Mam |
| Year Found | 2013 |
| Location Found | Nyack, NY USA |
| Finding Institution | Alliance University (formerly Nyack College) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.088552 N, 73.915076 W Map |
| Discovery Notes | Phage isolated, purified, and harvested by Christine Kline and Sarah Mam. Soil Type: Mud Color of Soil: Dark-brown Texture: Silt-like, wet Notes about Terrain, Habitat, and/or Structures Around Soil: Rocky, wet soil found along Hudson River (approx. 4 ft from shore during low tide). Soil was collected during low tide in an area that is normally submerged at high tide. EM picture taken by Brian E. Ford (Queens College) |
| Naming Notes | Christine E. Kline and Sarah E. Mam named this phage "TheHudson" because it was derived from soil in the Hudson River. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 20, 2013 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 11050 |
| Genome length (bp) | 50841 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGTGGTAA |
| GC Content | 64.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Duration of Incubation: 24 hrs Plaque Morphology: - Shape: Circular - Diameter: 2 - 2.5mm - Type of Phage: Lytic - Clarity of Plaque: Clear |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT359365 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.2x10^9 |
| Pitt Freezer Box# | 1 |
| Pitt Freezer Box Grid# | C3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
