The Actinobacteriophage Database

Mycobacterium phage Drake55

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Detailed Information for Phage Drake55
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Chloe Lindsey
Year Found	2016
Location Found	Bowling Green, KY USA
Finding Institution	Western Kentucky University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	30°C
GPS Coordinates	36.985139 N, 86.455417 W Map
Discovery Notes	The sample was taken next to the wall of a dorm building, near an exhaust pipe. Due to the close proximity of the site to a wall there is no foot traffic. There is no grass at the location. The soil’s texture was similar to that of clay. The sample was enriched using Mycobacterium smegmatis host cells and a first plaque was discovered on 9/28/16.
Naming Notes	The name of this phage is Drake55 because of my grandfather. His last name is Drake, and he drives and races a red 55' Chevrolet. I grew up watching him work on and perfect his 55', teaching me to not give up your dreams. Therefore I wanted to name the phage something memorable in his honor for being a great role model for me throughout the years.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 30, 2016
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	4228
Genome length (bp)	52719
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGTCGGTTA
GC Content	63.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	A
Subcluster	A2
Cluster Life Cycle	Temperate
Lysogeny Notes	The phage goes through the temperate life cycle.
Other Cluster Members	Click to View Chargerpower (None) Abbyshoes (A1) Abrogate (A1) Acme (A1) Adahisdi (A1) Aeneas (A1) AFIS (A1) Agaliana (A1) Ajay (A1) Alsfro (A1) Altman (A1) Alvin (A1) Anglerfish (A1) Applejack (A1) Arcanine (A1) Arlo (A1) Ashballer (A1) Atkinbua (A1) BaconJack (A1) Barriga (A1) BarrowTuph (A1) Beatrix (A1) BeesKnees (A1) Bethlehem (A1) Bexan (A1) Big3 (A1) Bigchungi (A1) Bigfoot (A1) BigMau (A1) BigPaolini (A1) BillKnuckles (A1) Bircsak (A1) Blue (A1) BluSpix (A1) Bob3 (A1) Bones (A1) BPBiebs31 (A1) Briton15 (A1) Bruns (A1) Burton (A1) Buttons (A1) Bxb1 (A1) CactusRose (A1) Carlyle (A1) Chanagan (A1) Ciao (A1) ConceptII (A1) Corvo (A1) Crispicous1 (A1) Cueylyss (A1) DD5 (A1) Dexes (A1) Doom (A1) Dreamboat (A1) DreamCatcher (A1) DrFeelGood (A1) Dulcie (A1) Dussy (A1) Dynamix (A1) Edtherson (A1) EnzoK (A1) Espresso (A1) Euphoria (A1) Eyeball (A1) Fajezeel (A1) Fascinus (A1) Fenn (A1) Forsytheast (A1) Francis47 (A1) Froghopper (A1) Fushigi (A1) GageAP (A1) Gandalf20 (A1) GMonster (A1) Gompeii16 (A1) Graduation (A1) GrecoEtereo (A1) Greg (A1) Gwendoluna (A1) Gyzlar (A1) Hami1 (A1) HanShotFirst (A1) HarryOW (A1) Hermia (A1) HermioneGrange (A1) Homines (A1) Hope4ever (A1) Ichabod (A1) IgnatiusPatJac (A1) ILeeKay (A1) Inyanga (A1) Iqorha (A1) JackSparrow (A1) Jasper (A1) JC27 (A1) Jerm2 (A1) Jorgensen (A1) JuliaChild (A1) Kanely (A1) KBG (A1) Kenmech (A1) Killigrew (A1) KSSJEB (A1) Kugel (A1) Kykar (A1) KyMonks1A (A1) Lamina13 (A1) Lesedi (A1) Levia (A1) Licorice (A1) LilBib (A1) Lockley (A1) Lopton (A1) LunarLander (A1) Magnar (A1) Magnito (A1) Makemake (A1) Manatee (A1) Marcell (A1) Marchy (A1) Marco3 (A1) Marge (A1) Maroc7 (A1) Marsha (A1) MaryBeth (A1) McGuire (A1) McSinger (A1) MetalQZJ (A1) Michley (A1) Mkhuseli (A1) Molly (A1) Monet (A1) Moose (A1) MPlant7149 (A1) MrGordo (A1) Mryolo (A1) Mule (A1) Museum (A1) Naira (A1) NEHalo (A1) Nerujay (A1) Nhonho (A1) Niza (A1) Norz (A1) Ohno789 (A1) Oogway (A1) PacerPaul (A1) Papez (A1) Paphu (A1) Paraselene (A1) Pari (A1) Parliament (A1) PascalRango (A1) PattyP (A1) Payneful (A1) Pelly (A1) Pepe (A1) Perseus (A1) Peterson (A1) Petp2012 (A1) Petruchio (A1) PherrisBueller (A1) PhineBark (A1) Phlippers (A1) PhrostyMug (A1) PinkPlastic (A1) Pinto (A1) Pippin (A1) Pita2 (A1) ProMouse (A1) PSullivan (A1) QTRlifeCrisis (A1) Raid (A1) Rajelicia (A1) Rhynn (A1) RidgeCB (A1) Ringer (A1) Rohr (A1) Rubeus (A1) Rufus (A1) Ruotula (A1) Rutherferd (A1) Sagefire (A1) Sandaddy (A1) Sanya (A1) SarFire (A1) Scowl (A1) Seabiscuit (A1) Seanderson (A1) ShortQueendom (A1) Sibs6 (A1) SkiPole (A1) Slagathor (A1) Smairt (A1) Smeagol (A1) Snazzy (A1) Solon (A1) Sorpresa (A1) SpikeBT (A1) Squee (A1) StewieG (A1) STLscum (A1) StrongArm (A1) Sumter (A1) Sunshine924 (A1) SwissCheese (A1) Switzer (A1) Swole (A1) Target (A1) Tasp14 (A1) Teodoridan (A1) TheloniousMonk (A1) Thor (A1) Topgun (A1) Tote (A1) Traft412 (A1) Treddle (A1) Tripl3t (A1) Trouble (A1) Turj99 (A1) TwoPeat (A1) U2 (A1) Violet (A1) Watermelon (A1) Wheeler (A1) Wilkins (A1) Zeeculate (A1) Zephyr (A1) Zeuska (A1) Chupacabra (A10) DontArgue (A10) Drake94 (A10) DustyMartin (A10) Eaglepride (A10) Edison31 (A10) Goose (A10) KittenMittens (A10) Kristoff (A10) OKCentral2016 (A10) PeaceMeal1 (A10) Poompha (A10) Rebeuca (A10) RhynO (A10) Rowdy (A10) Severus (A10) Shapes (A10) Topanga (A10) Trike (A10) Twister (A10) WalterMcMickey (A10) Aneem (A11) Bachome (A11) Bowtie (A11) Bud (A11) Ebony (A11) Et2Brutus (A11) Fibonacci (A11) Flaverint (A11) Gilberta (A11) Hutc2 (A11) Insomnia (A11) Jabith (A11) Joselito (A11) Lucivia (A11) Mabel (A11) MaCh (A11) Mulciber (A11) Munch (A11) Orange (A11) Petersenfast (A11) Salz (A11) Sham4 (A11) Snape (A11) Timothy (A11) TinyTimmy (A11) DarthPhader (A12) Pharaoh (A12) Refuge (A12) Steamy (A12) Zimmer (A12) Phlei (A13) Gail (A14) Jeeves (A14) Luchador (A14) SororFago (A14) Sunhee (A14) Anaysia (A15) Anon (A15) Battleship (A15) Boohoo (A15) DekHockey33 (A15) Epsocamisio (A15) JSwag (A15) KatherineG (A15) LastResort (A15) Looper (A15) MinecraftSteve (A15) Nebulosus (A15) Nedarya (A15) Oofda (A15) ReMo (A15) Remus (A15) Rosalind (A15) ShayRa (A15) Soups (A15) Strosahl (A15) Switzerland (A15) Waits (A15) Warrior24 (A15) EagleEye (A16) Lucyedi (A16) PainterBoy (A16) 40AC (A17) MyraDee (A18) Kimona (A19) 20ES (A2) AbbyPaige (A2) Acolyte (A2) Adzzy (A2) Agape74 (A2) AN3 (A2) AN9 (A2) ANI8 (A2) AnnaL29 (A2) Anselm (A2) ArcherNM (A2) BabyBack (A2) Bactobuster (A2) Baehexic (A2) BengiVuitton (A2) Benvolio (A2) BiancaTri92 (A2) BobSwaget (A2) Bradman (A2) Bugsy (A2) Caraxes (A2) Centaur (A2) Changeling (A2) Che12 (A2) ChipMunk (A2) Colin (A2) CRB1 (A2) Crucio (A2) D29 (A2) D32 (A2) Dalmatian (A2) DBQu4n (A2) Deloris (A2) Dignity (A2) Drake55 (A2) DudeLittle (A2) Duplo (A2) Echild (A2) Equemioh13 (A2) EvilGenius (A2) EZMoney23 (A2) Fameo (A2) FiringLine (A2) First (A2) Flare16 (A2) GaugeLDP (A2) Georgie2 (A2) Gratitude (A2) HarryHoudini (A2) Heffalump (A2) Herbertwm (A2) IronMan (A2) Iwokeuplikedis (A2) Jaan (A2) Jerm (A2) JoshKayV (A2) Journey13 (A2) Jsquared (A2) Kalpine (A2) Kerberos (A2) KingCyrus (A2) Koduck (A2) L5 (A2) LadyBird (A2) Landor (A2) Larenn (A2) Leogania (A2) Leviathan (A2) LilTurb (A2) LionsBait (A2) Lokk (A2) Loser (A2) MajorMajor (A2) Malec (A2) MiculUcigas (A2) Miko (A2) MissWhite (A2) Naji (A2) NaSiaTalie (A2) NicoleTera (A2) NothingSpecial (A2) Odin (A2) Ph8s (A2) Phaded (A2) Piro94 (A2) Pomar16 (A2) Power (A2) Pukovnik (A2) QueenB2 (A2) QueenBeesly (A2) Quokka (A2) Rachaly (A2) RedRock (A2) Retro23 (A2) SemperFi (A2) Serenity (A2) Sknot (A2) Smeagan (A2) SnapTap (A2) StarStuff (A2) Superchunk (A2) SweetiePie (A2) SwirlSquare (A2) TarsusIV (A2) Terrific (A2) TipsytheTRex (A2) Tomathan (A2) Travvers (A2) Tristan (A2) Trixie (A2) Trooper (A2) Turbido (A2) Updawg (A2) VA6 (A2) VC3 (A2) WeiHuaDA (A2) Whabigail7 (A2) WideWale (A2) XianYue (A2) Anthony (A20) FatCactus (A20) Kinmap (A21) ACFishhook (A3) Aglet (A3) AgronaGT15 (A3) Anubis (A3) AugsMagnumOpus (A3) BabyJohn (A3) BabyRay (A3) Beauxregard13 (A3) Bigflo (A3) BlueBird (A3) BreSam8 (A3) Bugatti (A3) BuzzBuzz (A3) Bxz2 (A3) Calvinny (A3) Caviar (A3) Chiqui (A3) Colbster (A3) Cullens (A3) DaHudson (A3) Daishi (A3) Dieselweasel (A3) DropBear (A3) EpicPhail (A3) Farber (A3) Fernando (A3) Fred313 (A3) Gemma (A3) GingkoMaracino (A3) Giroux (A3) Grif (A3) Groupthink (A3) Grub (A3) Grum1 (A3) GtownJaz (A3) HashRod (A3) HaveUMetTed (A3) Heathen (A3) HelDan (A3) Heliosoles (A3) Hercules11 (A3) Hookmount (A3) Idleandcovert (A3) Isca (A3) JenCasNa (A3) JeppNRM (A3) JF2 (A3) JF4 (A3) JHC117 (A3) Jobu08 (A3) Kachowdy (A3) KADY (A3) Kalb97 (A3) Kalnoky (A3) Lambert1 (A3) LarryKay (A3) LBerry (A3) Lilith (A3) Louie6 (A3) Lowa (A3) LugYA (A3) MA5 (A3) MadMarie (A3) Mainiac (A3) Malinsilva (A3) Manu (A3) Marchesa (A3) Margo (A3) Marie (A3) Marius (A3) MarQuardt (A3) Methuselah (A3) Microwolf (A3) Misomonster (A3) MK4 (A3) MoneyMay (A3) MuchMore (A3) Noella (A3) Norbert (A3) OlanP (A3) Ollie (A3) P28Green (A3) Panamaxus (A3) Pawn (A3) Pembroke (A3) Penny1 (A3) PGHhamlin (A3) Phantastic (A3) PhishRPhriends (A3) Phoebe (A3) Phoxy (A3) Phranny (A3) Pistachio (A3) Pocahontas (A3) Popcicle (A3) PotatoSplit (A3) Puppy (A3) PurpleHaze (A3) QuinnKiro (A3) Reba (A3) ResDef (A3) Rockstar (A3) Rummer (A3) Sabia (A3) Sabinator (A3) SaturnRing (A3) Scout (A3) Snickers (A3) SoilDragon (A3) Soshari (A3) SoYo (A3) Spike509 (A3) Stagni (A3) StepMih (A3) Taurus (A3) Texage (A3) Tiffany (A3) TinyPebbles (A3) TNguyen7 (A3) Todacoro (A3) Veracruz (A3) Vix (A3) Watson (A3) Wooldri (A3) Zetzy (A3) AbbysRanger (A4) Abdiel (A4) Achebe (A4) Albee (A4) Alberto7 (A4) Annyong (A4) Arturo (A4) AvatarAhPeg (A4) Avle17 (A4) Baby16 (A4) Backyardigan (A4) Badger (A4) BangNhom (A4) Bartimeaus (A4) BellusTerra (A4) Blackmoor (A4) Bombshell (A4) Broseidon (A4) Bruiser (A4) BubbleTrouble (A4) Bumblebee11 (A4) Burger (A4) Caelakin (A4) Camperdownii (A4) CentreCat (A4) Cerulean (A4) ChampagnePapi (A4) Chaph (A4) Cici (A4) Cindaradix (A4) Cintron (A4) Citius (A4) Clarenza (A4) Cocoaberry (A4) Commander (A4) Connomayer (A4) Datway (A4) Deano (A4) Dhanush (A4) DirtyDunning (A4) Druantia (A4) Eagle (A4) Eapen (A4) Eris (A4) Eros (A4) Eurydice (A4) Florean (A4) Flux (A4) Funston (A4) Gadost (A4) HamSlice (A4) Happiness (A4) Holli (A4) Houdini22 (A4) Huxley (A4) Iceman (A4) ICleared (A4) Iracema64 (A4) Jaykayelowell (A4) JetBlade (A4) JoongJeon (A4) Kampy (A4) Katalie136 (A4) KFPoly (A4) Kingmustik0402 (A4) Koan (A4) Koreni (A4) Kratark (A4) Kremtemulon (A4) Kyee (A4) LappelDuVide (A4) Lemur (A4) LeoAvram (A4) LHTSCC (A4) LittleB (A4) LittleGuy (A4) LochMonster (A4) Lorenzo (A4) Lunsford (A4) Maverick (A4) Maxo (A4) Mayonnaise (A4) Mazhar510 (A4) Medusa (A4) MeeZee (A4) Melvin (A4) Millski (A4) Miramae (A4) Morpher26 (A4) Morrow (A4) Mundrea (A4) Nebs (A4) Nemo27 (A4) Noelle (A4) NorthStar (A4) NotAPhaseMom (A4) Nyxis (A4) Obama12 (A4) Ohfah (A4) OKaNui (A4) Palestino (A4) Peaches (A4) Perplexer (A4) PeterPeter (A4) PetiteSangsue (A4) Phacado (A4) Phelipe (A4) Phighter1804 (A4) Phontbonne (A4) Pipcraft (A4) Polymorphads (A4) Pumbaa (A4) Relief (A4) Romney (A4) Roosevelt (A4) Rosa24 (A4) Ruin (A4) Sabertooth (A4) Scamp (A4) SenorClean (A4) Shaka (A4) SheldonCooper (A4) Shygu2 (A4) Skipitt (A4) Sparxx (A4) Spino (A4) Stasia (A4) Stink (A4) Taquarus (A4) Thanksgivukkah (A4) TinaFeyge (A4) Tinybot (A4) TiroTheta9 (A4) TroyPia (A4) TygerBlood (A4) Ulysses (A4) Wander (A4) Wilbur (A4) Wile (A4) Wizard007 (A4) Xena (A4) YoSam321 (A4) AgentM (A5) Airmid (A5) Aragog (A5) Archetta (A5) Benedict (A5) Bluefalcon (A5) Bonamassa (A5) Chadwick (A5) Conspiracy (A5) Coog (A5) Cuco (A5) Discoknowium (A5) Dublin (A5) ElTiger69 (A5) ForGetIt (A5) George (A5) Ghoulboy (A5) HuhtaEnerson15 (A5) Jabiru (A5) Jovo (A5) Lev2 (A5) LittleCherry (A5) MarysWell (A5) Micasa (A5) Midas2 (A5) Milcery (A5) Naca (A5) PetterN (A5) Phlorence (A5) PickleBack (A5) Prinashe11 (A5) Scorpia (A5) Swirley (A5) SydNat (A5) Tarynearal (A5) Theia (A5) Tiger (A5) Twigg (A5) UnionJack (A5) Zolita (A5) Artemis2UCLA (A6) BABullseye (A6) BlessJoy (A6) Blinn1 (A6) Blue7 (A6) Candra (A6) Chartreuse (A6) CloudWang3 (A6) Cookiedough (A6) DaVinci (A6) Dorothea (A6) EricB (A6) Garak (A6) Gladiator (A6) GreedyLawyer (A6) Gruunaga (A6) Hammer (A6) Helmet (A6) Hexamo (A6) Hoot (A6) Indra (A6) Isiphiwo (A6) Jeffabunny (A6) JewelBug (A6) Jordennis (A6) Kazan (A6) Kipper29 (A6) Koko (A6) Lilbunny (A6) McFly (A6) Neeharika16 (A6) Newrala (A6) Pmask (A6) Polyphemus (A6) Priamo (A6) Rifter (A6) Roksolana (A6) SmellyB (A6) SuperAwesome (A6) SuperCallie99 (A6) Temprado (A6) ToneTone (A6) Tucker (A6) VohminGhazi (A6) Wiks (A6) WunderPhul (A6) Yokurt (A6) Zaka (A6) Zulu (A6) DroogsArmy (A7) FlyCatcher (A7) HINdeR (A7) Sheen (A7) Timshel (A7) Toro (A7) Astro (A8) Danforth (A8) Dixon (A8) Expelliarmus (A8) Groundhog (A8) NearlyHeadless (A8) Phillis (A8) Roary (A8) Saintus (A8) Smeadley (A8) Stephig9 (A8) Aliter (A9) Alma (A9) Arissanae (A9) Beemo (A9) BogosyJay (A9) Catalina (A9) Charm (A9) Conquerage (A9) Darrell (A9) DreamTeam1 (A9) EdogawaKiddo (A9) Eidsmoe (A9) Elephantoon (A9) EmyBug (A9) ExplosioNervosa (A9) Fayely (A9) Hanray (A9) Holex (A9) HortumSL17 (A9) Jiawan (A9) Keziacharles14 (A9) Lilleskat (A9) LoneWolf (A9) Maminiaina (A9) Myxus (A9) Onglai (A9) PackMan (A9) Phaeder (A9) Phonnegut (A9) Pioneer (A9) Priya (A9) Qobbit (A9) Rahalelujah (A9) RyeScarlet (A9) Sachima (A9) Scherzo (A9) SheaKeira (A9) Spouty (A9) Toaka (A9) Tubs (A9) Ugenie5 (A9) Vanisoa (A9) Yecey3 (A9)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Original plaque picked for isolation was large turbid plaque approximately 5mm in diameter, in round 2 through round 4 a bullseye phage became prominent. The final purification was of a turbid plaque that was approximately 4.5mm in diameter. The plates were incubated for 48hrs at 30°C.
Morphotype	Siphoviridae
Number of Genes	96
Number of tRNAs	1
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Drake55_1 Drake55_2 Drake55_3 (VIP2-like toxin) Drake55_4 Drake55_5 (HNH endonuclease) Drake55_6 (terminase small subunit) Drake55_7 Drake55_8 Drake55_9 — tRNA (codon recognized: CAG; Cove Score: 45.19) Drake55_10 Drake55_11 (lysin A) Drake55_12 (holin) Drake55_13 (lysin B) Drake55_14 (terminase large subunit) Drake55_15 (portal protein) Drake55_16 (capsid maturation protease) Drake55_17 (scaffolding protein) Drake55_18 (major capsid protein) Drake55_19 (head-to-tail connector protein) Drake55_20 (head-to-tail adaptor) Drake55_21 (head-to-tail connector protein) Drake55_22 (head-to-tail stopper) Drake55_23 (head-to-tail connector protein) Drake55_24 (tail terminator) Drake55_25 (major tail protein) Drake55_26 (tail assembly chaperone) Drake55_27 (tail assembly chaperone) Drake55_28 (tape measure protein) Drake55_29 (minor tail protein) Drake55_30 (minor tail protein) Drake55_31 (minor tail protein) Drake55_32 (minor tail protein) Drake55_33 (minor tail protein) Drake55_34 Drake55_35 Drake55_36 (integrase) Drake55_37 Drake55_38 Drake55_39 Drake55_40 (excise) Drake55_41 Drake55_42 Drake55_43 Drake55_44 Drake55_45 Drake55_46 (DNA polymerase I) Drake55_47 Drake55_48 Drake55_49 (helix-turn-helix DNA-binding domain protein) Drake55_50 Drake55_51 (ThyX-like thymidylate synthase) Drake55_52 Drake55_53 (ribonucleotide reductase) Drake55_54 Drake55_55 Drake55_56 (sigma factor) Drake55_57 Drake55_58 (metallophosphoesterase) Drake55_59 Drake55_60 Drake55_61 (DNA primase) Drake55_62 (DNA primase) Drake55_63 Drake55_64 (endonuclease VII) Drake55_65 Drake55_66 (hydrolase) Drake55_67 Drake55_68 (helix-turn-helix DNA-binding domain protein) Drake55_69 Drake55_70 (DnaB-like dsDNA helicase) Drake55_71 Drake55_72 Drake55_73 Drake55_74 Drake55_75 (Cas4 exonuclease) Drake55_76 Drake55_77 (immunity repressor) Drake55_78 Drake55_79 Drake55_80 Drake55_81 Drake55_82 Drake55_83 Drake55_84 Drake55_85 Drake55_86 Drake55_87 Drake55_88 Drake55_89 Drake55_90 Drake55_91 Drake55_92 Drake55_93 Drake55_94 Drake55_95 Drake55_96 Drake55_97
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF668269
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Designator	2016WEKUDrake55CL
SEA Lysate Titer	7.7*10^6
Date of SEA Lysate Titering	Jan 11, 2016
Pitt Freezer Box#	37
Pitt Freezer Box Grid#	B9
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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