| Detailed Information for Phage Farber |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Josh Barber |
| Year Found | 2013 |
| Location Found | Uniontown, PA USA |
| Finding Institution | Seton Hill University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.866667 N, 79.716994 W Map |
| Discovery Notes | Farber was isolated from an enriched soil sample located near an old well. The soil was relatively moist and had an earthy scent. The sample was obtained and taken approximately 3cm into the dirt. Around the location are plenty of different plant species as well as concord-grape vines. |
| Naming Notes | "Farber" was a name that was given to me by a few friends in my first semester at Seton Hill University. They always kept me laughing and motivated and I wanted to dedicate this virus to my friends. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 1262 |
| Sanger Finishing Reactions | 7 |
| Genome length (bp) | 49190 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGTGGTAA |
| GC Content | 64.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Large plaque size (~3mm), turbid with halos. The phage took a while to grow, roughly 36 hours and with a lower concentration of agar. |
| Morphotype | Siphoviridae |
| Number of Genes | 84 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM233455 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.41x10^9 |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
E9 |
| Available Files |
| Final DNAMaster File | Download |