Mycobacterium phage Francis47
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Liberone (GI) Neuvillette (FR) FatCactus (A20) Rossetti (L2) RoseMarie (None)Mycobacterium phage Francis47
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| Detailed Information for Phage Francis47 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Natalie Macy |
| Year Found | 2017 |
| Location Found | Marietta, GA USA |
| Finding Institution | University of North Georgia |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.9127 N, 84.6135 W Map |
| Discovery Notes | The soil sample was collected in a flower garden. There were small flowers, bushes, grass, and wood chips. The flower garden was planted over a year ago. It was sunny and the temperature was 28.88 degrees Celsius. The soil was moist, consisted of potting soil, and was regularly water. |
| Naming Notes | I named my phage after my grandfather, Glen. His middle name is Francis and the year he was born was 1947. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 4, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3302 |
| Genome length (bp) | 53936 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.6% |
| Sequencing Notes | The reads from this sample showed several deletions were present in some subset of the population. It's not clear whether the three different sections were deleted at once, but there was no evidence that the second and third deletions occurred together. That is, either the second deletion happened, or the third, or neither, but not both 2 and 3 in the same population. About 20% of the reads indicate a 2964 bp deletion from 26691 to 29654, inclusive. About 15% of the reads indicate a 449 bp deletion from 50614 to 51062, inclusive. About 15% of the reads indicate a 563 bp deletion from 51008 to 51570, inclusive. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Alliance University (formerly Nyack College) |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque is relatively large at 10mm. The plaque has one big circle, and surrounding it is a halo. The halo is 5mm. The circle in the middle is cloudy, and the outline of the circle is defined. The halo surrounding the circle is even more defined. |
| Morphotype | Siphoviridae |
| Number of Genes | 93 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524497 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Ignore |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |