| Detailed Information for Phage Happiness |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Parker Niccum |
| Year Found | 2021 |
| Location Found | FAIRFAX, VA United States |
| Finding Institution | George Mason University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 29°C |
| GPS Coordinates | 38.828796 N, 77.307118 W Map |
| Discovery Notes | Isolated from moist, clumping dirt, 2 inches below surface. Sampled approximately 10 feet away from a large campus building. |
| Naming Notes | The plaque which was ultimately used to create the large volume lysate was part of a group of plaques that formed a 'smiley face', complete with eyes and a nose. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 25, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1114 |
| Genome length (bp) | 51398 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Sequencing Notes | 1% of reads mapping to Cluster EG |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | George Mason University |
| Annotation Status | In GenBank |
| Plaque Notes | Capsid width: 64.71 nm
Tail length: 147.06 nm |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP297529 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
139 |
| Pitt Freezer Box Grid# |
D5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
