| Detailed Information for Phage HarryHoudini |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jenna Christman and Kate Ossello |
| Year Found | 2016 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 47.667778 N, 117.39806 W Map |
| Discovery Notes | Found from a soil sample outside of the Gonzaga bookstore on the Gonzaga University campus. The soil came from soil surrounding a drainage ditch. |
| Naming Notes | The reason for the name comes from the fact that our phage kept failing to show up on plates because it is a temperate phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 13, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 116 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 52722 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.4% |
| Sequencing Notes | This sequence was generated as part of a DOGEMS sample and later identified via PCR as belonging to the phage HarryHoudini.
There is a single SNP in the sequence. At position 25047, ~45% of the reads show a T there instead of the majority-called G. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were turbid and perfectly round. The size varied depending on the length of time. Our phage is not from the clusters KF or N, and it can grow at 37 degrees. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OM203159 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^6 |
| Date of SEA Lysate Titering |
Nov 4, 2016 |
| Pitt Freezer Box# |
40 |
| Pitt Freezer Box Grid# |
F8 |
| Available Files |
| GenBank File for Phamerator | Download |